Comprehensive search of diagnostic biomarker for autism based on mass analysis of peripheral blood and birth cohort study by cord blood

在这个项目中，我们正在执行分析，以寻找特定的蛋白质或分析物的质量分析使用的血清自闭症。然后，我们有一个计划，以确定候选标记分子的基础上，通过脐带血出生队列自闭症的早期诊断。在蛋白质组学分析中，我们期望通过双向电泳进行差异显示，但由于设备故障而失败。在代谢组学分析中，我们发现，与对照组相比，孤独症患者血清中游离脂肪酸和酰基肉毒碱增加，从而得出结论，孤独症患者血清中的脂解正在进行。本补助期内尚未检查脐带血出生队列。

In this project, we were executing analysis to search specific protein or analyte by mass analysis using serum of individuals with autism. Then, we had a plan to identify the candidate marker molecule for early diagnosis of autism based on the birth cohort by cord blood. In proteomics analysis, we expected to execute differential display by two-dimensional electrophoresis but failed because of the equipment disorder. In metabolome analysis, we identified that free fatty acid and acyl-carnitine were increased in serum of individuals with autism as compared controls, which concluded that lipolysis was progressing in serum of individuals with autism. The birth cohort by cord blood have not yet examined within this term of grant-in-aid.