Morphology and function of mossy fiber sprouting associated with limbic seizures

苔藓纤维发芽可能发挥的作用，边缘癫痫发作的泛化和表达进行了检查。本研究采用杏仁核点燃大鼠作为慢性癫痫模型。采用杏仁核内注射二丁酰环腺苷酸（ab-cAMP）诱导的边缘系统癫痫模型。采用E1小鼠作为遗传性癫痫模型。在点燃大鼠，形态学检查显示苔藓纤维发芽的证据，在内部分子层的齿状回和在CA 3/CA 4子字段的双边campi。边缘癫痫持续状态引起了类似的发芽，特别是在双侧齿状回。然而，在El小鼠中没有获得苔藓纤维发芽的证据。因此，点燃和癫痫持续状态是研究癫痫相关苔藓纤维发芽的合适模型，而El小鼠不是.当经历边缘癫痫持续状态的大鼠进行MAP 2免疫组织化学，在MAP 2免疫反应性显着增加，观察到在双侧齿状回。提示边缘系统损伤后齿状回苔藓纤维突触发生重组. Sprague-Dawley（SD）大鼠海马CA 3/CA 4区可见锥体下束苔藓纤维。这种苔藓纤维的分布在Wistar大鼠中未见，但在点燃完成后变得明显。损毁颗粒细胞可降低SD大鼠点燃率，但对Wistar大鼠无影响。点燃完成后，同样的处理抑制了Wistar大鼠的全身点燃癫痫发作，但对SD大鼠没有影响。提示锥体下区苔藓纤维在边缘系统运动性发作的发生发展中起重要作用。

The possible roles played by mossy fiber sprouting in the generalization and expression of limbic seizures were examined. In this study, amygdaloid-kindled rat was used as a chronic model of epilepsy. Limbic status epileptics induced by intra-amygdaloid injection of dibutyryl-cAMP (ab-cAMP) was used an acute model of epilepsy. El mouse was used as a hereditary model of epilepsy.1. In kindled rats, morphological examination showed evidence of mossy fiber sprouting in the inner molecular layer of the dentate gyrus and in the CA3/CA4 subfield of bilateral hippocampi. Limbic status epileptics caused a similar sprouting, especially in the bilateral dentate gyri. However, no evidence of mossy fiber sprouting was obtained in El mice. Thus, kindling and status epilepticus, but not El mouse, are suitable models for examination of seizure-related mossy fiber sprouting.2. When rats which experienced limbic status epilepticus were subjected to MAP2 immunohistochemistry, a marked increase in MAP2 immunoreactivity was observed in the bilateral dentate gyri. This suggest that mossy fiber synaptic reorganization occurs in the dentate gyri after limbic seizures.3. Sprague-Dawley (SD) rats have infrapyramidal bundle of mossy fibers in CA3/CA4 subfield of the hippocampus. This distribution of mossy fibers is not seen in Wistar rats, but becomes to be evident after completion of kindling. Destruction of granule cells decreased the rate of kindling in SD rats, but had no effect in Wistar rats. The same treatment after completion of kindling suppressed generalized kindled seizures in Wistar rats, but had no effect in SD rats. It is suggested that mossy fibers in the infrapyramidal region have an important role in the development of limbic motor seizures.