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Morphology and function of mossy fiber sprouting associated with limbic seizures

与边缘癫痫发作相关的苔藓纤维发芽的形态和功能

基本信息

批准号：
03670569
负责人：
MORI Norio
金额：
$ 0.96万
依托单位：
Fukushima Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670569/
关键词：
epileptic seizures hippocampus mossy fiber granule cell sprouting けいれん発作

项目摘要

The possible roles played by mossy fiber sprouting in the generalization and expression of limbic seizures were examined. In this study, amygdaloid-kindled rat was used as a chronic model of epilepsy. Limbic status epileptics induced by intra-amygdaloid injection of dibutyryl-cAMP (ab-cAMP) was used an acute model of epilepsy. El mouse was used as a hereditary model of epilepsy.1. In kindled rats, morphological examination showed evidence of mossy fiber sprouting in the inner molecular layer of the dentate gyrus and in the CA3/CA4 subfield of bilateral hippocampi. Limbic status epileptics caused a similar sprouting, especially in the bilateral dentate gyri. However, no evidence of mossy fiber sprouting was obtained in El mice. Thus, kindling and status epilepticus, but not El mouse, are suitable models for examination of seizure-related mossy fiber sprouting.2. When rats which experienced limbic status epilepticus were subjected to MAP2 immunohistochemistry, a marked increase in MAP2 immunoreactivity was observed in the bilateral dentate gyri. This suggest that mossy fiber synaptic reorganization occurs in the dentate gyri after limbic seizures.3. Sprague-Dawley (SD) rats have infrapyramidal bundle of mossy fibers in CA3/CA4 subfield of the hippocampus. This distribution of mossy fibers is not seen in Wistar rats, but becomes to be evident after completion of kindling. Destruction of granule cells decreased the rate of kindling in SD rats, but had no effect in Wistar rats. The same treatment after completion of kindling suppressed generalized kindled seizures in Wistar rats, but had no effect in SD rats. It is suggested that mossy fibers in the infrapyramidal region have an important role in the development of limbic motor seizures.

研究了苔藓纤维发芽在边缘系统癫痫发作的泛化和表达中可能发挥的作用。在这项研究中，杏仁核点燃的大鼠被用作癫痫的慢性模型。杏仁核内注射二丁酰-cAMP（ab-cAMP）诱导的边缘系统癫痫持续状态被用作癫痫的急性模型。采用El小鼠作为癫痫遗传模型。 1．在点燃的大鼠中，形态学检查显示在齿状回的内分子层和双侧海马的 CA3/CA4 亚区有苔藓纤维发芽的证据。边缘系统癫痫持续状态引起类似的发芽，特别是在双侧齿状回。然而，在 El 小鼠中没有获得苔藓纤维发芽的证据。因此，点燃和癫痫持续状态，但不是El小鼠，是检查癫痫相关苔藓纤维发芽的合适模型。2．当经历边缘癫痫持续状态的大鼠接受 MAP2 免疫组织化学检查时，在双侧齿状回中观察到 MAP2 免疫反应性显着增加。这表明边缘叶癫痫发作后齿状回发生了苔藓纤维突触重组。3． Sprague-Dawley (SD) 大鼠的海马 CA3/CA4 亚区有锥体束下的苔藓纤维。这种苔藓纤维的分布在 Wistar 大鼠中未见，但在点燃完成后变得明显。颗粒细胞的破坏降低了 SD 大鼠的点燃率，但对 Wistar 大鼠没有影响。点燃完成后的相同治疗抑制了 Wistar 大鼠的全身点燃性癫痫发作，但对 SD 大鼠没有效果。有人认为锥体下区域的苔藓纤维在边缘运动性癫痫发作的发展中具有重要作用。