Development of novel detection system of circulating tumor cells with tumor-specific replication adenovirus and its clinical application for predicting the recurrence of ovarian cancer

新型肿瘤特异性复制腺病毒循环肿瘤细胞检测系统的开发及其预测卵巢癌复发的临床应用

• 批准号: 23659775
• 负责人: KYO SATORU
• 金额: $ 2.25万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659775/
• 关键词: CTC; gynecological cancer; ovarian cancer; telomerase; adenovirus; GFP; 末梢血中循環癌細胞; 卵巣癌; 血中循環がん細胞; 婦人科癌; 増殖型アデノウイルス; 末梢血中循環がん細胞; テロメレース

We established the novel detection system for circulating tumor cells (CTC) with telomerase-specific replication adenovirus in which GFP cDNA was integrated for GFP fluorescence. Infection of these viruses with 5ml of blood samples from the patients with gynecological cancers successfully identified CTC, while no CTC was detectd in the samples from healthy volunteers. While the number or the presence of CTCs were not significantly correlated with clinicopathological characteristics, they were tightly related to the treatment outcome as well as the overall survival. Thus, the present study clearly found the clinical utility of this novel detection system for CTC.

我们用端粒酶特异性复制腺病毒建立了用于循环肿瘤细胞（CTC）的新型检测系统，其中将GFP cDNA整合为GFP荧光。 这些病毒从妇科癌症患者中成功鉴定出CTC的患者感染了5ML的血液样本，而在健康志愿者的样本中未发现CTC。 尽管CTC的数量或存在与临床病理学特征没有显着相关，但它们与治疗结果以及总体生存率密切相关。 因此，本研究清楚地发现了该新型CTC检测系统的临床实用性。

项目成果

A novel CTC detection technique and its clinical application

一种新型CTC检测技术及其临床应用

• 作者: 高倉正博;京 哲
• 通讯作者: 京 哲

子宮頸癌における腫瘍特異的増殖型アデノウイルス末梢血中腫瘍細胞の単離と遺伝子解析

宫颈癌肿瘤特异性增殖腺病毒外周血肿瘤细胞的分离及基因分析

• 发表时间: 2012
• 作者: 高倉正博;京哲;中村充宏;毎田佳子;水本泰成;保野由紀子;張英智;藤原俊義;井上正樹
• 通讯作者: 井上正樹

Plasma membrane proteomics identifies bone marrow stromal antigen 2 as a potential therapeutic target in endometrial cancer

• DOI: 10.1002/ijc.27679
• 发表时间: 2013-01-15
• 期刊: INTERNATIONAL JOURNAL OF CANCER
• 影响因子: 6.4
• 作者: Yokoyama, Takuhei;Enomoto, Takayuki;Naka, Tetsuji
• 通讯作者: Naka, Tetsuji

子宮頸癌における末梢血中腫瘍細胞の単離と単一細胞からの遺伝子解析

宫颈癌外周血肿瘤细胞的分离及单细胞遗传分析

• 发表时间: 2012
• 作者: 高倉正博;京哲;中村充宏;毎田佳子;水本泰成;保野由紀子;井上正樹
• 通讯作者: 井上正樹

Circulating tumor cells detected by a novel adenovirus-mediated system may be a potent therapeutic marker in gynecologic cancers.

通过新型腺病毒介导的系统检测到的循环肿瘤细胞可能是妇科癌症的有效治疗标志物。

• 发表时间: 2012
• 期刊: Br.J.Cancer.
• 作者: Yonezawa R;Wada T;Morita M;Sawakawa K;Ishii Y;Sasahara M;Tsuneki H;Sasaoka T;Saito S.;Masahiro Takakura
• 通讯作者: Masahiro Takakura