Development of novel detection system of circulating tumor cells with tumor-specific replication adenovirus and its clinical application for predicting the recurrence of ovarian cancer

我们建立了一种新的检测循环肿瘤细胞（CTC）的端粒酶特异性复制腺病毒，其中绿色荧光蛋白（GFP）的cDNA整合的荧光。 用这些病毒感染妇科恶性肿瘤患者的5ml血样，成功地检测出CTC，而在健康志愿者的血样中未检测到CTC。 虽然CTC的数量或存在与临床病理特征没有显著相关性，但它们与治疗结果以及总生存期密切相关。 因此，本研究清楚地发现了这种新型CTC检测系统的临床实用性。

We established the novel detection system for circulating tumor cells (CTC) with telomerase-specific replication adenovirus in which GFP cDNA was integrated for GFP fluorescence. Infection of these viruses with 5ml of blood samples from the patients with gynecological cancers successfully identified CTC, while no CTC was detectd in the samples from healthy volunteers. While the number or the presence of CTCs were not significantly correlated with clinicopathological characteristics, they were tightly related to the treatment outcome as well as the overall survival. Thus, the present study clearly found the clinical utility of this novel detection system for CTC.