Micro RNA expression profile in lungs of the autopsied patients with amniotic fluid embolism

本研究的目的是探讨羊水栓塞组和非羊水栓塞组尸检肺组织中miRNA表达谱的差异。我们用福尔马林固定石蜡包埋的三例羊水栓塞尸检患者的肺组织和一例非羊水栓塞尸检患者的肺组织作为对照。从福尔马林固定的石蜡包埋肺组织中提取RNA。用A260(核酸)/A280(蛋白质)比值对提取的RNA进行质量评价。然后用东丽miRNA寡核苷酸芯片分析miRNA的表达谱。羊水栓塞组肺组织中5种miRNAs的表达强度是非羊水栓塞组的1.5倍以上。相反，羊水栓塞组肺组织中12miRNAs的表达强度比非羊水栓塞组降低了1.5倍以上。

The aim of this study was to evaluate the difference of micro RNA (miRNA) expression profiles between in lungs of the autopsied patients with amniotic fluid embolism and in those with non-amniotic fluid embolism. We used formalin fixed paraffin embedded lung tissues of the three autopsied patients with amniotic fluid embolism and one autopsied patient with non-amniotic fluid embolism as a control. RNA was extracted from formalin fixed paraffin embedded lung tissues. The qualities of extracted RNA were evaluated by using A260(nucleic acid)/A280(protein) ratio. Then, miRNA expression profiles were analysed by Toray miRNA Oligo Chip. In lung tissues of the patients with amniotic fluid embolism, intensities of five miRNAs increased more than 1 1/2 times than in those with non-amniotic fluid embolism. Contrary,in lung tissues of the patients with amniotic fluid embolism, intensities of12 miRNAs decreased more than 1 1/2 times than in those with non-amniotic fluid embolism.