Clarification of survival strategy and host recognition mechanism of root parasitic weeds

根寄生杂草生存策略及寄主识别机制的阐明

• 批准号: 15380079
• 负责人: YONEYAMA Koichi
• 金额: $ 10.18万
• 依托单位: Utsunomiya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380079/
• 关键词: root parasitic weeds; germination stimulants; strigolactone; LC; MS; host recognition mechanism; carotenoid biosynthesis; P450 monooxygenase; ABC transporter; 吸器誘導物質; エチレン生合成; DNAチップ; カロテノイド生合成阻害剤; 植物ホルモン

Characterization of strigolactones, germination stimulants for root parasitic weeds, Striga and Orobanche, exuded by their host plants was conducted. One of the major strigolactones of monocotyledonous plants, maize, millet and sorghum, was found to be deoxystrigol which is known as a host recognition signal for arbuscular mycorrhizal (AM) fungi. On the other hand, one of the major strigolactones of dicotyledonous plants was identified as orobanchol. In addition to these known strigolactones, at least 10 novel strigolactones were found in plant species examined. Non-mycorrhizal plants, such as spinach and white lupin, and Japanese red pine tree were found to exude strigolactones. These results indicate that strigolactones are distributed widely in the plant kingdom and other factors as well as strigolactones appear to be involved in host recognition of AM fungi.Through LC/MS/MS analyses of orobanchol in the root exudates, it was first demonstrated that in red clover plants reduced supply of phosphorus but not of other mineral nutrients examined (N, K, Mg, Ca) in the culture medium significantly promoted the release of orobanchol by the root of the plant. In addition, 10 nM fluridone, an inhibitor of carotenoid biosynthesis, reduced orobanchol exudation markedly, supporting the hypothesis that strigolactones are synthesized via carotenoids in planta. This carotenoid biosynthetic inhibitor alone elicited seed germination of Striga asiatica and also induced haustorium-like morphogenesis in the germinated seeds.DNA microarray analyses of Arabidopsis thaliana treated with strigol suggested that several genes including two P450 genes and ABC transporter gene are potentially involved in the biosynthesis and metabolism of strigolactones.Further study on biosynthesis and metabolism of strigolactones should shed light to both symbiotic and parasitic interactions in the rhizosphere.

对根寄生杂草独脚金和列当的萌发促进剂独脚金内酯的性质进行了研究。脱氧独脚金醇是单子叶植物玉米、小米和高粱中的主要独脚金内酯之一，是AM真菌识别寄主的信号分子。另一方面，双子叶植物的主要独脚金内酯之一被鉴定为列当醇。除了这些已知的独脚金内酯，至少有10个新的独脚金内酯被发现在植物物种检查。非菌根植物，如菠菜和白色羽扇豆，日本赤松被发现分泌独脚金内酯。这些结果表明独脚金内酯在植物界中广泛分布，并且其他因素以及独脚金内酯似乎参与了AM真菌对寄主的识别.通过对根分泌物中列当醇的LC/MS/MS分析，首次证明了在红三叶草植物中，磷的供应减少，而其他矿质营养的供应没有被检测到（N、K、Mg、Ca）对列当醇的释放有显著的促进作用。此外，10 nM氟啶酮，类胡萝卜素生物合成的抑制剂，减少列当醇渗出显着，支持独脚金内酯通过类胡萝卜素在植物中合成的假设。这种类胡萝卜素生物合成抑制剂单独引起独脚金种子萌发，也诱导吸器，通过对独脚金处理的拟南芥的DNA微阵列分析表明，独脚金内酯的生物合成和代谢可能与P450基因和ABC转运蛋白基因有关，对独脚金内酯生物合成和代谢的进一步研究将为独脚金内酯的生物合成和代谢研究提供新的思路。共生和寄生的相互作用在根际。

项目成果

Germination response of Orobanche seeds subjected to conditioning temperature, water potential and growth regulator treatments

• DOI: 10.1111/j.1365-3180.2005.00477.x
• 发表时间: 2005-12
• 期刊: Weed Research
• 影响因子: 1.7
• 作者: W. Song;W. Zhou;Z. Jin;D. Cao;D. Joel;Y. Takeuchi;K. Yoneyama

Interaction between Orobanche crenata and its host legumes:: unsuccessful haustorial penetration and necrosis of the developing parasite

• DOI: 10.1093/aob/mci105
• 发表时间: 2005-05-01
• 期刊: ANNALS OF BOTANY
• 影响因子: 4.2
• 作者: Pérez-De-Luque, A;Rubiales, D;Joel, DM
• 通讯作者: Joel, DM

Effects of fluridone and norflurazon on conditioning and germination of Striga asiatica seeds

• DOI: 10.1007/s10725-005-4746-5
• 发表时间: 2006-01-01
• 期刊: PLANT GROWTH REGULATION
• 影响因子: 4.2
• 作者: Kusumoto, D;Chae, SH;Takeuchi, Y
• 通讯作者: Takeuchi, Y

Search for germination stimulants and inhibitors for root parasitic weeds.

寻找根寄生杂草的发芽刺激剂和抑制剂。

• 期刊: American Chemical Society Symposium Series 927
• 作者: Yoneyama;K.;Sate;D.;Takeuchi;Y;Sekimoto;H.;Yokota;T.;Sassa;T.
• 通讯作者: T.

Interaction between Orobanche crenate and its host legumes : unsuccessful haustorial penetration and necrosis of the developing parasite.

列当圆齿与其宿主豆科植物之间的相互作用：吸器渗透不成功和发育中的寄生虫坏死。

• 发表时间: 2005
• 期刊: Annals of Botany 95
• 作者: Perez-de-Luque;A.;Rubiales;D.;Cubero;J.I.;Press;M.C.;Scholes;J.;Yoneyama;K.;Takeuchi;Y.;Plakhine;D.;Joel;D.M.
• 通讯作者: D.M.