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Study on novel drug-resistant bacteria in oral biofilms and the resistance mechanisms

口腔生物膜中新型耐药菌及其耐药机制研究

基本信息

批准号：
23659878
负责人：
KOKEGUCHI Susumu
金额：
$ 2.41万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2012
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659878/
关键词：
細菌感染症歯学薬剤耐性バイオフィルム

项目摘要

The continuing emergence of multi-drug resistance in Pseudomonas, Acinetobacter, and Mycobacterium tuberculosisis a global health problem. Recently Dr. Dantas G et al. reported that their isolated hundreds of soil bacteria had the capacity to grow on antibiotics as a sole carbon source. Many bacteria subsisting on antibiotics were closely related to human pathogens. The aim of this study was to investigate the distribution of the multi-drug resistant bacteria and bacteria subsisting on antibiotics in dental plaques and dental environments such as dental unit water lines. Representative isolates capable of growth on penicillin and kanamycin were isolated from Dental unit water lines. They were identified Sphingomonas aquatilisa n d Chitinophaga sp. by 16S ribosomal RNA gene sequencing analysis. No multi-drug resistant bacteria and bacteria subsisting on antibiotics were detected in any of the samples from healthy subjects and patients with periodontitis. However, drug resistant Enterococcus faecalis, methicillin resistant Staphylococcus epidermidis and β-lactam antibiotics resistant oral Streptococci were isolated from the oral cavity of a patient with long-term systemic antibiotic therapy for severe bacterial infection(septis). Dental plaques may alter their composition and enrich for bacteria resistant to antibiotics by systemic antibiotic therapy and bacteria subsisting on antibiotics have been also present in dental clinic environment.

假单胞菌、不动杆菌和结核分枝杆菌的多重耐药性的持续出现是一个全球性的健康问题。最近，Dantas G博士等人报告说，他们分离出的数百种土壤细菌能够在抗生素作为唯一碳源的情况下生长。许多依赖抗生素生存的细菌与人类病原体密切相关。本研究的目的是调查多重耐药菌和抗生素生存的细菌在牙菌斑和牙科环境中的分布，如牙科综合治疗台水管。能够在青霉素和卡那霉素上生长的代表性分离株是从牙科综合治疗台水管中分离的。经16 S核糖体RNA基因序列分析，鉴定为水生鞘氨醇单胞菌（Sphingomonasaquatilisa）和噬几丁虫属（Chitinophagasp.）在健康受试者和牙周炎患者的任何样本中均未检测到多重耐药菌和抗生素生存菌。然而，耐药粪肠球菌、耐甲氧西林表皮葡萄球菌和耐β-内酰胺类抗生素口腔链球菌是从一名因严重细菌感染（败血症）接受长期全身抗生素治疗的患者口腔中分离出来的。通过全身抗生素治疗，牙菌斑可改变其组成并富集对抗生素耐药的细菌，并且在牙科临床环境中也存在以抗生素存活的细菌。