Development of virus-free generation and xeno-free culture of human gingiva-derived iPS cells

iPS细胞有望成为再生治疗的有力工具；然而，在临床应用之前需要解决的问题包括病毒载体衍生的插入诱变和使用动物来源的饲养细胞进行培养。我们用附着体质粒载体生成了人牙龈成纤维细胞（hGF）衍生的iPS细胞。所得的iPS细胞克隆可在hGF饲养细胞上维持。因此，hGF衍生的iPS细胞的无病毒和自体培养是iPS细胞未来治疗用途的重要一步。

iPS cells are expected to be a powerful tool for regenerative treatments; however, problems that need to be addressed prior to clinical application include viral-vector-derived insertional mutagenesis and the use of animal-derived feeder cells for culture. We generated human gingival fibroblast (hGF)-derived iPS cells with episomal plasmid vectors. The resulting iPS cell clones could be maintained on hGF feeder cells. The virus-free and autologous culture of hGF derived-iPS cells thus represents an important step toward the future therapeutic usage of iPS cells.