Development of cell-based screening system for identifying osteogenesis-targeting compounds

有效影响破骨细胞发生或成骨的小分子化合物可能构成牙槽骨再生医学有效治疗策略的基础。在本研究中，我们建立了基于破骨细胞祖细胞（NFAT/荧光素酶报告细胞）或成骨细胞（I型胶原/GFP报告细胞）的高通量筛选系统。这些筛选系统可以以可靠的方式识别破骨细胞生成或成骨靶向化合物的候选物，从而使其成为寻找新的骨形成合成调节因子的有前途的工具。

Small-molecule compounds that potently affect osteoclastogenesis or osteogenesis could form the basis for effective therapeutic strategies for alveolar bone regenerative medicine. In this study, we established osteoclast progenitor cell (NFAT/luciferase reporter cell)- or osteoblast (type I collagen/GFP reporter cell)-based high-throughput screening systems. These screening systems could identify candidates for osteoclastogenesis- or osteogenesis-targeting compounds in reliable manner, thus making it a promising tool for finding novel synthetic regulators of bone formation.