Development of stem cell therapy for the oral tissues damaged by chemo-radiation therapy

开发针对放化疗损伤口腔组织的干细胞疗法

• 批准号: 23659952
• 负责人: ASAHINA Izumi
• 金额: $ 2.33万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659952/
• 关键词: 口腔粘膜炎; 細胞治療; 骨髄由来細胞; 放射線障害; 唾液腺萎縮; 口腔癌; 放射線

Salivary gland atrophy or osteonecrosis are serious side effects for patients with head and neck cancer who received radiation- and/or chemo-therapy. It causes the harmful effects on the quality of life after cancer treatment. However, current clinical strategies cannot efficiently prevent the occurrence of these side effects. Therefore, effectivetherapy should be developed urgently. Recently, we have shown that cell therapy with bone marrow derived cells (BMDCs) displays the preventive effects to the radiation-induced salivary gland hypofunction. Therefore, to develop this strategy, we examined the detailed condition of cell transplantation in mouse model that has radiation-induced salivary gland atrophy and osteonecrosis. As results, both intra-grandular or intra-venous injections of BMDCs, and both young and older mouse donors were as effective in repairing irradiated salivary gland. However, though the cultured mesenchymal stem cells (MSCs) were also effective to rescue the salivary hypofunction by its injection via tail vein, the survival rate of mice was seriously decreased. Cultured MSCs seemed to cause the acute pulmonary embolism after injection.

对于接受放疗和/或化疗的头颈癌患者来说，唾液腺萎缩或骨坏死是严重的副作用。它会对癌症治疗后的生活质量产生有害影响。然而，目前的临床策略无法有效预防这些副作用的发生。因此，迫切需要开发有效的治疗方法。最近，我们已经证明，骨髓来源细胞（BMDC）的细胞疗法对辐射引起的唾液腺功能低下具有预防作用。因此，为了制定这一策略，我们检查了具有辐射诱导唾液腺萎缩和骨坏死的小鼠模型中细胞移植的详细情况。结果，无论是粒内注射还是静脉注射 BMDC，以及年轻和年老的小鼠供体，在修复受辐射的唾液腺方面都同样有效。然而，虽然通过尾静脉注射培养的间充质干细胞（MSCs）也能有效挽救唾液功能低下的小鼠，但小鼠的存活率却严重下降。培养的间充质干细胞注射后似乎会引起急性肺栓塞。

项目成果

細胞治療による唾液腺再生療法に関する基礎的研究

细胞疗法唾液腺再生疗法的基础研究

• 发表时间: 2012
• 作者: 住田吉慶;Tran SD;各務秀明;朝比奈泉
• 通讯作者: 朝比奈泉

放射線性の口腔内障害に対する骨髄由来幹細胞治療応用の検討

骨髓干细胞治疗放射性口腔病变的应用探讨

• 发表时间: 2012
• 作者: 井隆司;住田吉慶;大場誠悟;南里篤太郎;朝比奈泉
• 通讯作者: 朝比奈泉

Over view on regenerative therapy for alveolar bone (Keynote lecture2)

牙槽骨再生治疗概述（主题演讲2）

• 发表时间: 2011
• 作者: Shinozaki S;Moriyama;M;Hayashida J-N;Tanaka A;Maehara T;Ieda S;Nakamura S;Asahina I
• 通讯作者: Asahina I

Alveolar bone regeneration using mesenchymal stem cells

使用间充质干细胞进行牙槽骨再生

• 发表时间: 2010
• 作者: Moriyama M;Hayashida J-N;Toyoshima T;Ohyama Y;Shinozaki S;Tanaka A;Maehara T;Nakamura S;Asahina I
• 通讯作者: Asahina I

Predictive factor for photodynamic therapy effects on oral squamous cell carcinoma and oral epithelial dysplasia.

• DOI: 10.1016/j.archoralbio.2011.04.012
• 发表时间: 2011-11
• 期刊: Archives of oral biology
• 影响因子: 3
• 作者: M. Uehara;H. Ikeda;M. Nonaka;Y. Sumita;A. Nanashima;T. Nonaka;I. Asahina