DEVELOPMENT OF CELL HYBRID ARTIFICIAL BONE USING 3-D CELL CULTURE SYSTEM

(1)骨形态发生蛋白（BMP）和碱性成纤维细胞生长因子（bFGF）对骨髓细胞的影响：我们研究了BMP和bFGF对大鼠骨髓细胞的影响，发现BMP刺激细胞增殖和分化，而bFGF刺激细胞增殖但抑制细胞分化。特别是bFGF促进了骨祖细胞的增殖，这些细胞尚未分化为成熟的成骨细胞。因此，在成骨细胞分化的早期阶段bFGF的处理增加了成骨细胞的分化。成骨细胞的数量和后期BMP的处理诱导成骨细胞的分化。结果，在成骨细胞分化的最佳阶段，BMP和bFGF的治疗促进了骨生成。(2)细胞杂交人工骨的制备：将骨髓细胞植入大鼠皮下，在多孔羟基磷灰石中培养，诱导新骨形成。在体外培养过程中，BMP和/或BMP的处理增强了体内骨形成。我们还研究了多孔聚（糖，乳酸共聚物）作为三维细胞培养的支架，但由于它的疏水性，细胞没有渗透到块深。

(1) Effects of Bone Morphogenetic Protein (BMP and basic Fibroblast Growth Factor (bFGF) on Bone Marrow Cel1s : We examined the effects of BMP and bFGF on rat bone marrow cells, and revealed that BMP stimulated both cell proliferation and differentiation, while bFGF stimulated cell proliferation but inhibited cell differentiation. bFGF, especially, enhanced the proliferation of osteoprogenitor cells, which had not diffeientiated into matures osteoblast yet. Therefor, the treatment of bFGF at the early stage of osteoblastic differentiation increased the. number of osteoprpgenitor cells and the treatment of BMP at the later stage induced differentiation of osteoblast. As a result the treatment of both BMP and bFGF at the optimal stage of osteoblastic differentiation promoted osteogenesis.(2) Fabrication of Cell Hybrid Artificial Bone : New bone formation was induced in rat subcutaneous tissue by implanted bone marrow cells cultured in porous hydroxyapatite. The treatment of BMP and/or bEOF during the culture in vitro enhanced bone formation in vivo. We also examined porous poly (gycoic, lactic acid co-polymer) as a scaffold for 3-D cell culture, but cells did not infiltrate deeply into the block because of it7s hydrophobicity.