DEVELOPMENT OF CELL HYBRID ARTIFICIAL BONE USING 3-D CELL CULTURE SYSTEM

(1)骨形态发生蛋白（Bone Morphogenetic Protein， BMP）和碱性成纤维细胞生长因子（basic Fibroblast Growth Factor, bFGF）对骨髓细胞的影响：我们研究了BMP和碱性成纤维细胞生长因子（bFGF）对大鼠骨髓细胞的影响，发现BMP促进细胞增殖和分化，bFGF促进细胞增殖但抑制细胞分化。特别是bFGF对尚未分化为成熟成骨细胞的成骨细胞增殖有促进作用。因此，在成骨细胞分化早期使用bFGF治疗，可提高成骨细胞的成骨能力。成骨细胞数量和后期BMP处理诱导成骨细胞分化。结果表明，在成骨细胞分化的最佳阶段，BMP和bFGF均能促进成骨。(2)细胞杂交人工骨的制备：利用多孔羟基磷灰石培养的骨髓细胞植入大鼠皮下组织诱导新生骨形成。在体外培养过程中，BMP和/或bEOF的处理促进了体内骨的形成。我们还研究了多孔聚乙二醇，乳酸共聚物作为3d细胞培养的支架，但由于其7s疏水性，细胞没有深入渗透到块中。

(1) Effects of Bone Morphogenetic Protein (BMP and basic Fibroblast Growth Factor (bFGF) on Bone Marrow Cel1s : We examined the effects of BMP and bFGF on rat bone marrow cells, and revealed that BMP stimulated both cell proliferation and differentiation, while bFGF stimulated cell proliferation but inhibited cell differentiation. bFGF, especially, enhanced the proliferation of osteoprogenitor cells, which had not diffeientiated into matures osteoblast yet. Therefor, the treatment of bFGF at the early stage of osteoblastic differentiation increased the. number of osteoprpgenitor cells and the treatment of BMP at the later stage induced differentiation of osteoblast. As a result the treatment of both BMP and bFGF at the optimal stage of osteoblastic differentiation promoted osteogenesis.(2) Fabrication of Cell Hybrid Artificial Bone : New bone formation was induced in rat subcutaneous tissue by implanted bone marrow cells cultured in porous hydroxyapatite. The treatment of BMP and/or bEOF during the culture in vitro enhanced bone formation in vivo. We also examined porous poly (gycoic, lactic acid co-polymer) as a scaffold for 3-D cell culture, but cells did not infiltrate deeply into the block because of it7s hydrophobicity.