DEVELOPMENT OF CELL HYBRID ARTIFICIAL BONE USING 3-D CELL CULTURE SYSTEM

使用 3-D 细胞培养系统开发细胞混合人工骨

• 批准号: 08557114
• 负责人: ASAHINA Izumi
• 金额: $ 4.93万
• 依托单位: TOKYO MEDICAL AND DENTAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08557114/
• 关键词: ARTIFICIAL BONE; BMP; FGF; BONE MARROW CELL; HYDROXYAPATITE; ハイブリッド; bFGF; 再生; 成長因子

(1) Effects of Bone Morphogenetic Protein (BMP and basic Fibroblast Growth Factor (bFGF) on Bone Marrow Cel1s : We examined the effects of BMP and bFGF on rat bone marrow cells, and revealed that BMP stimulated both cell proliferation and differentiation, while bFGF stimulated cell proliferation but inhibited cell differentiation. bFGF, especially, enhanced the proliferation of osteoprogenitor cells, which had not diffeientiated into matures osteoblast yet. Therefor, the treatment of bFGF at the early stage of osteoblastic differentiation increased the. number of osteoprpgenitor cells and the treatment of BMP at the later stage induced differentiation of osteoblast. As a result the treatment of both BMP and bFGF at the optimal stage of osteoblastic differentiation promoted osteogenesis.(2) Fabrication of Cell Hybrid Artificial Bone : New bone formation was induced in rat subcutaneous tissue by implanted bone marrow cells cultured in porous hydroxyapatite. The treatment of BMP and/or bEOF during the culture in vitro enhanced bone formation in vivo. We also examined porous poly (gycoic, lactic acid co-polymer) as a scaffold for 3-D cell culture, but cells did not infiltrate deeply into the block because of it7s hydrophobicity.

(1)骨形态发生蛋白（BMP）和碱性成纤维细胞生长因子（bFGF）对骨髓细胞的影响：我们研究了BMP和bFGF对大鼠骨髓细胞的影响，发现BMP刺激细胞增殖和分化，而bFGF刺激细胞增殖但抑制细胞分化。尤其是碱性成纤维细胞生长因子（bFGF）可促进尚未分化为成熟成骨细胞的骨祖细胞的增殖。因此，在成骨细胞分化的早期阶段bFGF的处理增加了成骨细胞的分化。成骨细胞数量和后期BMP处理诱导成骨细胞分化。结果，在成骨细胞分化的最佳阶段，BMP和bFGF的治疗促进了骨生成。(2)细胞杂交人工骨的制备：将骨髓细胞植入大鼠皮下，在多孔羟基磷灰石中培养，诱导新骨形成。在体外培养过程中，BMP和/或BMP的处理增强了体内骨形成。我们还研究了多孔聚（糖，乳酸共聚物）作为三维细胞培养的支架，但由于它的疏水性，细胞没有渗透到块深。

项目成果

S Oida,et.al: "Biological Activities of Bone Extract containing TGF-β superfamily proteins" J.Hard Tiss.Biol.7・1. 27-36 (1998)

S Oida等人：“含有TGF-β超家族蛋白的骨提取物的生物活性”J.Hard Tiss.Biol.7·1（1998）。

Isobe M and Yamazaki Y Oida S,Ishihara K., Nakabayashi N,Amagasa, T.: "Bone morphogenetic protein encapsulated with a biodegradable and biocompatible polymer." J.Biomed.Mater.Res.32. 433-438 (1996)

Isobe M 和 Yamazaki Y Oida S、Ishihara K.、Nakabayashi N、Amagasa、T.：“用可生物降解和生物相容性聚合物封装的骨形态发生蛋白。”

Laczka-Osyczka A.Laczka M.Kasugai S.Ohya K.: "Behavior of bone marrow cells cultured on three different coatings of gel-derived bioactive glass-ceramics at early stages of cell differentiation." Journal of Biomedical Materials Research.42 (3). 433-442 (19

Laczka-Osyczka A.Laczka M.Kasugai S.Ohya K.：“在细胞分化早期阶段，在三种不同的凝胶衍生生物活性玻璃陶瓷涂层上培养的骨髓细胞的行为。”

Morotome Y,Goseki-Sone M,Ishikawa I,Oida S: "Gene expression of growth and differentiation factor-5, -6, -7 in developing bovine tooth at the root forming stage" Biochm.Biophys.Res.Comm.244. 85-90 (1998)

Morotome Y、Goseki-Sone M、Ishikawa I、Oida S：“生长和分化因子-5、-6、-7 在牛牙牙根形成阶段的基因表达”Biochm.Biophys.Res.Comm.244。

Y.Morotome.et al: "Gene Expression of Growth and Differetiation Factors-S,-6,and-7 in Developing Bovine Tooth at the Root Forming stage" Biochemical and Biophysical Research Communications.224・1. 85-90 (1998)

Y.Morotome.et al：“在牙根形成阶段发育中的生长和分化因子-S、-6和-7的基因表达”生物化学和生物物理研究通讯。224・1（1998）。