Identification of in vitro reproductive toxicants in house and office environments

室内粉尘是包括阻燃剂(FRS)在内的多种污染物的水槽。为了揭示室内粉尘的高优先级终点和污染物候选物质，我们使用基于人U2OS细胞系的报告基因分析，表征了从日本和国外收集的室内粉尘粗提物和23个选定的FR的内分泌干扰能力。室内粉尘提取物对Era、GR和PPARg2有拮抗作用，对AR、PR、GR和PPARg2有拮抗作用。大多数FR仅对ERA和PPARg2具有激动性，部分FR对所有受试核受体均表现出拮抗作用。系统聚类清楚地表明ERA的激动性和AR和PR的拮抗性是常见的。鉴于我们之前关于室内粉尘中FRS浓度的结果，以及我们目前的结果，这些影响的候选贡献者包括FRS，如TDCIPP。本研究建立的TDCIPP体内生殖毒性检测方法刚刚起步。

Indoor dust is a sink for many kinds of pollutants, including flame retardants (FRs). To reveal high-priority endpoints of and contaminant candidates in indoor dust, using reporter gene assays based on human U2OS cell lines, we characterized the endocrine-disrupting potencies of crude extracts of indoor dust collected from Japan and abroad and for 23 selected FRs. Indoor dust extracts were agonistic to ERa, GR, and PPARg2 and antagonistic against AR, PR, GR, and PPARg2. A majority of FRs was agonistic to ERa and PPARg2 only, and some FRs demonstrated antagonism against all tested nuclear receptors. Hierarchical clustering clearly indicated agonism of ERa and antagonism of AR and PR were common. Given our previous results regarding the concentrations of FRs in indoor dust and in light of our current results, candidate contributors to these effects includes FRs such TDCIPP. We have just started in vivo reproductive toxicity testing for TDCIPP by testing method established in this study.