Dynamics of polarisome and ROS generating complex during pathogenic development of fungi

本研究探讨了NADPH氧化酶(NOx)在寄主穿透过程中的作用机制。在黑斑病中，存在两种NOX同系物，NOXA和NOXB，只有NOXB参与了寄主的穿透。因此，我们对NOX(S)的表达模式和定位进行了研究。NOx的表达在孢子萌发过程中被诱导。NoxB蛋白定位于附着胞。NoxB的破坏导致附着胞内肌动蛋白积累的紊乱。此外，NoxB还与raca相互作用。RACA的中断突变体受到多个菌丝生长阶段的影响，失去致病性。

This study was evaluated the functional mechanism of NADPH oxidase (Nox) during host penetration. In black spot disease, two Nox homologues, NoxA and NoxB are present and only the NoxB was involved in host penetration. Therefore, we evaluated the expression pattern and localization of Nox(s). The expression of Nox were induced during spore germination. NoxB protein was localized at the appressorium. The disruption of noxB resulted in disorder of actin accumulation at the appressorium. Moreover, NoxB was interacted with RacA. The disruption mutant of racA was affected multiple hyphal growth stages and lost pathogenicity.