Start-up study of the structural biology of cellulose synthase complex

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Electron microscopy was used for the three-dimensional structural determination of cellulose synthase complex. Preliminary analysis showed a three-dimensional model with some features. Our model is too primitive to be analyzed in detail, but it was shown as a result that the first X-ray crystallographic structure of cellulose synthase, which was reported in 2013 by another group, fits well to our structural model. We thus believe that our strategy is available for the structural biology of cellulose synthase, which will be used for future studies.We also conducted, for discussing the structural data, some functional analyses of cellulose synthase: kinetics of crude enzyme and functional reconstitution of recombinant cellulose synthase in E. coli. Especially the latter result allows the functional analysis to be done with recombinant protein. We designated this system as CESEC (CEllulose Synthesis in E. Coli), which will be a promising tool for studying cellulose synthase.