The role of FGF signal in brain development

FGF信号在大脑发育中的作用

• 批准号: 23700410
• 负责人: SATO Tatsuya
• 金额: $ 2.83万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23700410/
• 关键词: 神経発生; 形態形成; 神経回路形成; 脳; 神経; 発生; 神経回路

It was shown that the combination of in vivo electroporation andthe newest version of the tetracycline (Tet)-controlled gene regulatory (Tet-On) systemefficiently can induce gene expression in various types of neurons such as cerebralpyramidal neurons in mouse embryonic and postnatal tissues. Fate map analysis showedthat the Fgf8-expressing cells differentiate into the cerebellum. The marked cells aredetected in more posterior and lateral part of the cerebellum when the Fgf8-expressingcells are marked in earlier developmental stage.

结果表明，体内电穿孔和最新版本的四环素（泰特）控制的基因调控（Tet-On）系统相结合，可以有效地诱导小鼠胚胎和出生后组织中各种类型神经元（如大脑锥体神经元）的基因表达。命运图分析显示Fgf 8表达细胞向小脑分化。Fgf 8阳性细胞在发育早期多见于小脑后部和外侧部。

项目成果

Inducible gene expression in postmitotic neurons by an in vivo electroporation-based tetracycline system

• DOI: 10.1016/j.jneumeth.2013.01.014
• 发表时间: 2013-04
• 期刊: Journal of Neuroscience Methods
• 影响因子: 3
• 作者: Tatsuya Sato;Yuko Muroyama;Tetsuichiro Saito

Functional analysis of Nepro, a gene required for the maintenance of neocortex neural progenitor cells

Nepro 的功能分析，Nepro 是维持新皮质神经祖细胞所需的基因

• 发表时间: 2011
• 作者: Tatsuya Sato;Yuko Muroyama and Tetsuichiro Saito
• 通讯作者: Yuko Muroyama and Tetsuichiro Saito

Nepro is required for the maintenance of neural progenitor cells in the early neocortex

Nepro 是维持早期新皮质神经祖细胞所必需的

• 发表时间: 2012
• 作者: Tetsuichiro Saito