Development of conditional expression system in mice generated by site-specific gene integration method

点击翻译按钮获取中文摘要

The aim of this study is to make our targeted transgenesis method (PITT) more versatile by developing a conditional and tissue-specific expression system applicable to it. For this purpose, we conducted: 1) generation of PITT mice exhibiting tissue-specific transgene expression, 2) development of Dre reporter mouse useful for evaluation of Dre expression, 3) development of new seed mouse line that allows to integrate floxed allele, and 4) development of inducible expression system using KRAB repressor.