Microfluidic device for cellular assay of various samples

利用微型引脚对侧壁进行机械描述的可重构侧壁可移动微流控通道，在通道内输送难流动物体方面具有优势，可用于分析各种分析物/样品。然而，该装置在针的多次进料以及将通道中的流体吸入间隙方面存在问题。我们开发了一种探针，可以将单个针加热到较高的温度，并通过摩擦给它们提供动力，还有一种密封胶，它的粘度会随着温度的变化而急剧变化。我们发现，聚烯烃与二氧化硅或氟树脂填料的混合物减少了热粘度下降，而它显著降低粘度仅在加热针附近。我们还成功地在一个通道内绘制了不同配方的丙烯酰胺的多个凝胶块。

Reconfigurable microfluidic channel with movable sidewalls by mechanically descritizing the sidewalls using miniature pins has advantages in the transporting difficult-to-flow objects within the channels, and thus it is useful in analyzing various analytes/samples. The device, however, has problems in multiple feeding of the pins, and suctioning the fluid in the channel into the gaps. We have developed a probe that heats single pins to an elevated temperature and that feeds them with friction, and a sealant that changes its viscosity steeply with the temperature. We found a mixure of polyolefins with silica or fluororesin fillers reduced the viscosity drop by heat while it significantly lower the viscosity only near the heated pin. We also successfully patterned multiple gel blocks of different formulation of acrylamide in line within one channel.