Identification of CTL epitope using mass spectrometer.

细胞毒性T淋巴细胞（CTL）通过识别与细胞表面人类白细胞抗原（HLA）结合的肿瘤抗原衍生肽发挥抗肿瘤作用。为了鉴定肿瘤抗原的不同CTL表位，检查了基于质谱的方法。为了研究肿瘤特异性抗原提呈机制中的CTL表位组，将突变的K-ras基因导入MCF 10A细胞。结果，基因修饰的MCF 10A细胞具有组成型活性自噬体并产生自噬体依赖性表位。这些数据表明，具有肿瘤特异性抗原呈递机制的细胞为我们提供了通过该机制处理的各种CTL表位的信息。

Cytotoxic T lymphocytes (CTLs) exert anti-tumor effects through recognition of tumor antigen-derived peptides bound to human leukocyte antigens (HLAs) on cell surfaces. To identify diverse CTL epitopes of tumor antigens, mass spectrometry-based approach are examined. To investigate CTL epitope panel through tumor-specific antigen presenting machinery, MCF10A cells were transduced mutated K-ras gene. As a result, the gene-modified MCF10A cells had constitutively active autophagosomes and produced autophagosome-dependent epitopes. These data suggest that the cells having tumor-specific antigen presenting machinery provide us information on various CTL epitopes processed through the machinery.