Identification of the cell type specific region for retroviral vector integration

逆转录病毒载体在LMO2启动子附近的整合是基因治疗试验中白血病发生的最可能原因。在这里，我们发现了一个高发病率区域（HIR）的逆转录病毒载体整合，我们最初确定在LMO2启动子区域，靠近CCND2基因座的启动子区域，这表明有HIR在启动子区域的各种基因。为了对HIR进行全面分析，应用下一代测序仪检查HIR的分析条件。此外，使用下一代测序仪，鉴定了600个原癌基因或细胞增殖相关基因的候选基因，它们是HIR上载体整合的靶。

Retroviral vector integration near the LMO2 promotor is the most likely cause of leukemogenesis in the gene therapy trails. Here, we showed identification of a high incidence region (HIR) for retroviral vector integration, which we had originally identified in the LMO2 promotor region, near the promotor region of the CCND2 locus, suggesting that there is the HIR in the promotor region of various genes. To perform comprehensive analysis of HIR, next-generation sequencer was applied to examine the analysis condition for the HIR. Furthermore, using the next-generation sequencer, 600 candidates for proto-oncogene or the cell proliferation-related gene, which are targets for the vector integration on HIR, were identified.