Identification of the cell type specific region for retroviral vector integration

在基因治疗中，LMO2启动子附近的逆转录病毒载体整合是最可能导致白血病发生的原因。在这里，我们发现了一个逆转录病毒载体整合的高发区（HIR），我们最初在LMO2启动子区域发现，靠近CCND2位点的启动子区域，这表明在各种基因的启动子区域都存在HIR。为了对HIR进行全面分析，采用新一代测序仪对HIR的分析条件进行检测。此外，利用下一代测序仪，确定了600个候选原癌基因或细胞增殖相关基因，这些基因是HIR载体整合的靶标。

Retroviral vector integration near the LMO2 promotor is the most likely cause of leukemogenesis in the gene therapy trails. Here, we showed identification of a high incidence region (HIR) for retroviral vector integration, which we had originally identified in the LMO2 promotor region, near the promotor region of the CCND2 locus, suggesting that there is the HIR in the promotor region of various genes. To perform comprehensive analysis of HIR, next-generation sequencer was applied to examine the analysis condition for the HIR. Furthermore, using the next-generation sequencer, 600 candidates for proto-oncogene or the cell proliferation-related gene, which are targets for the vector integration on HIR, were identified.