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Engineering of monomer supplying enzyme for enhanced biopolymer production

Engineering of monomer supplying enzyme for enhanced biopolymer production
用于增强生物聚合物生产的单体供应酶工程
批准号:
23710102
负责人:
WATANABE Tsuyoshi
金额:
$2.83万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012

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项目成果

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中文摘要
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英文摘要
Acetoacetyl-CoA reductase (PhaB) catalyzes the reduction of acetoacetyl-CoA into 3-hydroxubutyryl-CoA using NADPH as a reducing power.3-Hydroxybutyryl-CoA serves as a monomer substrate for the microbial polyester, polyhydroxybutyrate. The aim of this study is to engineer PhaB for enhancing the enzymatic activity. The kinetic analysis of the selected mutants revealed that they possess enhanced turnover rate. The effectiveness of the obtained mutants of PhaB on polyhydroxybutyrate production was evaluated using Corynebacterium glutamicum, indicating that the content of polyhydroxybutyrate was increased up to 3-fold compared to the wild-type PhaB.
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動力学的解析および立体構造に基づいた進化型アセトアセチル-CoAレダクターゼの高活性化メカニズムの解明
基于动力学分析和三维结构阐明进化乙酰乙酰辅酶A还原酶的高激活机制
DOI: --
发表时间: 2013
期刊:
影响因子: --
作者: [渡辺剛志, 松本謙一郎, 本橋廉, 池田弘二, 田中良和, 姚閔, 田中勲, 田口精一]
通讯作者: 田口精一
Improvement of polyhydroxybutyrate production by the engineered ecetoacetyl-CoA reductase in Corynebacterium glutamicum
谷氨酸棒杆菌中工程化的乙酰乙酰辅酶A还原酶改善聚羟基丁酸的生产
DOI: --
发表时间: 2012
期刊:
影响因子: --
作者: [Tsuyoshi Watanabe, Yuyang Song, Ken'ichiro Matsumoto, Toshihiko Ooi, Seiichi Taguchi]
通讯作者: Seiichi Taguchi
PHA生合成系におけるモノマー供給酵素アセトアセチル-CoAレダクターゼの高活性変異体の酵素特性
PHA生物合成系统中单体供应酶乙酰乙酰辅酶A还原酶高活性突变体的酶特性
DOI: --
发表时间: 2011
期刊:
影响因子: --
作者: [渡辺剛志, 早坂知士, 池田弘二, 本橋廉, 松本謙一郎, 田口精一]
通讯作者: 田口精一
動力学的解析および立体構造に基づいた進化形アセトアセチル-CoAレダクターゼの高活性化メカニズムの解明
基于动力学分析和三维结构阐明进化乙酰乙酰辅酶A还原酶的高激活机制
DOI: --
发表时间: 2013
期刊:
影响因子: --
作者: [渡辺剛志, 松本謙一郎, 本橋廉, 池田弘二, 田中良和, 姚閔, 田中勲, 田口精一]
通讯作者: 田口精一
8
    The impacts of short term climate variability on Neanderthal - Homo sapiens replacement deduced from coral records(Fostering Joint International Research)
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      15KK0145
    • 项目类别:
      Fund for the Promotion of Joint International Research (Fostering Joint International Research)
    • 资助金额:
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    • 财政年份:
      2016
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      WATANABE Tsuyoshi
    • 依托单位:
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    • 批准号:
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    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $11.23万
    • 财政年份:
      2015
    • 负责人:
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    • 依托单位:
    Diversity in the global organization of the Golgi apparatus in differentiated secretory cells.
    • 批准号:
      26460263
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.16万
    • 财政年份:
      2014
    • 负责人:
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    • 依托单位:
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    • 批准号:
      25257207
    • 项目类别:
      Grant-in-Aid for Scientific Research (A)
    • 资助金额:
      $28.29万
    • 财政年份:
      2013
    • 负责人:
      WATANABE Tsuyoshi
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