Engineering of monomer supplying enzyme for enhanced biopolymer production
Engineering of monomer supplying enzyme for enhanced biopolymer production
批准号:
23710102
负责人:
WATANABE Tsuyoshi
金额:
$2.83万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012
中文摘要
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英文摘要
Acetoacetyl-CoA reductase (PhaB) catalyzes the reduction of acetoacetyl-CoA into 3-hydroxubutyryl-CoA using NADPH as a reducing power.3-Hydroxybutyryl-CoA serves as a monomer substrate for the microbial polyester, polyhydroxybutyrate. The aim of this study is to engineer PhaB for enhancing the enzymatic activity. The kinetic analysis of the selected mutants revealed that they possess enhanced turnover rate. The effectiveness of the obtained mutants of PhaB on polyhydroxybutyrate production was evaluated using Corynebacterium glutamicum, indicating that the content of polyhydroxybutyrate was increased up to 3-fold compared to the wild-type PhaB.
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動力学的解析および立体構造に基づいた進化型アセトアセチル-CoAレダクターゼの高活性化メカニズムの解明
基于动力学分析和三维结构阐明进化乙酰乙酰辅酶A还原酶的高激活机制
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[渡辺剛志, 松本謙一郎, 本橋廉, 池田弘二, 田中良和, 姚閔, 田中勲, 田口精一]
通讯作者:
田口精一
Improvement of polyhydroxybutyrate production by the engineered ecetoacetyl-CoA reductase in Corynebacterium glutamicum
谷氨酸棒杆菌中工程化的乙酰乙酰辅酶A还原酶改善聚羟基丁酸的生产
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Tsuyoshi Watanabe, Yuyang Song, Ken'ichiro Matsumoto, Toshihiko Ooi, Seiichi Taguchi]
通讯作者:
Seiichi Taguchi
PHA生合成系におけるモノマー供給酵素アセトアセチル-CoAレダクターゼの高活性変異体の酵素特性
PHA生物合成系统中单体供应酶乙酰乙酰辅酶A还原酶高活性突变体的酶特性
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[渡辺剛志, 早坂知士, 池田弘二, 本橋廉, 松本謙一郎, 田口精一]
通讯作者:
田口精一
動力学的解析および立体構造に基づいた進化形アセトアセチル-CoAレダクターゼの高活性化メカニズムの解明
基于动力学分析和三维结构阐明进化乙酰乙酰辅酶A还原酶的高激活机制
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[渡辺剛志, 松本謙一郎, 本橋廉, 池田弘二, 田中良和, 姚閔, 田中勲, 田口精一]
通讯作者:
田口精一
アセトアセチルCoAレダクターゼ高活性変異体の解析とそれを用いたCorynebacterium glutamicumによるPHB生産の増強
乙酰乙酰辅酶A还原酶高活性突变体的分析及利用该突变体增强谷氨酸棒杆菌PHB生产
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[渡辺剛志, 宋育陽, 松本謙一郎, 大井俊彦, 田口精一]
通讯作者:
田口精一
共 8 条
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Characteristics in the overall organization and dynamics of the Golgi apparatus in the anterior pituitary endocrine cells.
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High resolution reconstruction of past earthquakes and tsunami during last several hundreds using coral skeletons collected from Sumatra
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Pliocene El Nino -High-Resolution Coral Evidence of Robust Interannual Variability During Warm Period
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Effects of LHRH analogues on the ultrastructure of male rat pituitary gonadotropes
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cDNA microarray analyses on the gene expression in the pituitary glands under the physiological and experimental conditions.
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Pathophysiological Roles of Peroxisome Proliferator Activated Receptor (PPAR) on Development and Progression of Chronic kidney diseases -Search for a New Therapeutic Target for Chronic Kidney Diseases-
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Analyses on the secretory granule formation by using a yeast two hybrid system
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MOLECULAR MECHANISMS OF ACTION AND PHYSIOLOGICAL ROLES OF LIPID AUTACOIDS IN THE NEPHRON
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Ultrastructure analysis of germinal nucleus and its differentiation to the vegetative nucleus in Paramecium bursaria.
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Development of easy and quantitative reverse transcription-polymerase chain reaction method (MRT-PCR) for minute clinical samples and its clinical applications
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Cellular and molecular biological study on the pathophysiological roles of receptors for lipid mediators on atharosclerosis
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"The Speciation and the Hybridization of Sulawesi Macaques.
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Biochemical Study on Leukoriene D_4 Receptor in the Lung
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Purification and physiological-patho physiological significance of renal prostaglandin E_2 receptor.
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