Development of optically-driven tool for nano-bio processing and its applications in molecular dissection of genomic DNA

纳米生物加工光学驱动工具的开发及其在基因组DNA分子解剖中的应用

• 批准号: 23710145
• 负责人: TERAO Kyohei
• 金额: $ 2.91万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23710145/
• 关键词: 分子マニピュレーション; 分子加工; DNA; 分子操作; 光ピンセット; ゲノム; バイオナノテクノロジー; 一分子操作; ＭＥＭＳ; マイクロＴＡＳ

In this research project, we developed a novel nano/micro-fabricated tool for processing single biomolecule for the application in the research field of genome analysis. The technique for fabricating nano/micro structures was developed, which enables us to control the visibility of the structures under a fluorescence microscope. Their characteristics including the yield of fabrication and the trapping force were evaluated. We also verified the immobilization of proteins on their surface by surface plasmon resonance sensing. The results suggest the successful protein immobilization and the functionalization for a protein-modified nano/micro tool.

在本研究计划中，我们开发了一种新型的纳米/微米加工工具，用于处理单个生物分子，用于基因组分析研究领域。开发了用于制造纳米/微米结构的技术，这使得我们能够在荧光显微镜下控制结构的可见性。对它们的制作成品率和捕获力等特性进行了评价。我们还验证了固定化的蛋白质在其表面上的表面等离子体共振传感。结果表明，成功的蛋白质固定和功能化的蛋白质修饰的纳米/微米工具。

项目成果

Optically-Driven Microstructures for Manipulating Single DNA Molecule under FluorescenceMicroscope

荧光显微镜下操纵单个 DNA 分子的光驱动微观结构

• 发表时间: 2012
• 作者: C. Masuda;K. Terao;H. Oana;M. Washizu;T. Suzuki;H. Takao;F. Shimokawa;F. Oohira
• 通讯作者: F. Oohira

Optically-Driven Microstructures for Manipulating Single DNA Molecule under Fluorescence Microscope

荧光显微镜下操纵单个 DNA 分子的光驱动微观结构

• 发表时间: 2012
• 作者: C. Masuda;K. Terao;H. Oana;M. Washizu;T. Suzuki;H. Takao;F. Shimokawa;F. Oohira
• 通讯作者: F. Oohira

Size-exclusion SPR sensor chip: application to detection of aggregation and disaggregation of biological particles

• DOI: 10.1039/c2an16010b
• 发表时间: 2012-01-01
• 期刊: ANALYST
• 影响因子: 4.2
• 作者: Terao, Kyohei;Shimizu, Kazunori;Oohira, Fumikazu
• 通讯作者: Oohira, Fumikazu

DNA1 分子操作に用いる光駆動微小構造体の特性評価

用于 DNA1 分子操纵的光驱动微结构的表征

• 发表时间: 2011
• 作者: 増田千洋;寺尾京平;鈴木孝明;高尾英邦;下川房男;小穴英廣;鷲津正夫;大平文和
• 通讯作者: 大平文和

Localized substance delivery to single cell and 4D imaging of its uptake using a flow channel with a lateral aperture

• DOI: 10.1007/s10404-011-0885-3
• 发表时间: 2012-01-01
• 期刊: MICROFLUIDICS AND NANOFLUIDICS
• 影响因子: 2.8
• 作者: Terao, Kyohei;Okonogi, Atsuhito;Kotera, Hidetoshi
• 通讯作者: Kotera, Hidetoshi