Development of optically-driven tool for nano-bio processing and its applications in molecular dissection of genomic DNA

在本研究计划中，我们开发了一种新型的纳米/微米加工工具，用于处理单个生物分子，用于基因组分析研究领域。开发了用于制造纳米/微米结构的技术，这使得我们能够在荧光显微镜下控制结构的可见性。对它们的制作成品率和捕获力等特性进行了评价。我们还验证了固定化的蛋白质在其表面上的表面等离子体共振传感。结果表明，成功的蛋白质固定化和功能化的蛋白质修饰的纳米/微米工具。

In this research project, we developed a novel nano/micro-fabricated tool for processing single biomolecule for the application in the research field of genome analysis. The technique for fabricating nano/micro structures was developed, which enables us to control the visibility of the structures under a fluorescence microscope. Their characteristics including the yield of fabrication and the trapping force were evaluated. We also verified the immobilization of proteins on their surface by surface plasmon resonance sensing. The results suggest the successful protein immobilization and the functionalization for a protein-modified nano/micro tool.