Functional characterisation of a gene family encoding circadian regulated glycine-rich RNA-binding proteins in Arabidopsis thaliana
拟南芥中编码昼夜节律调节的富含甘氨酸的 RNA 结合蛋白的基因家族的功能表征
基本信息
- 批准号:5413633
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Grants
- 财政年份:2003
- 资助国家:德国
- 起止时间:2002-12-31 至 2013-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The overall goal of this project is a functional characterisation of the clock-controlled RNA-binding proteins AtGRP7 (Arabidopsis thaliana glycine-rich RNA-binding protein) and AtGRP8. AtGRP7 is part of a negative feedback loop through which it regulates oscillations of its own transcript as well as of the atgrp8 transcript. We suggest that the AtGRP7 feedback loop is involved in transmitting timing signals generated by a central circadian clock to clock-controlled processes within the cell. To define the function of this feedback loop we propose to identify target transcripts of AtGRP7. Since such transcripts are expected to show an altered expression in transgenic plants constitutively overexpressing AtGRP7 (AtGRP7-ox), transcript profiles will be compared between wild type plants and AtGRP7-ox plants employing DNA microarrays. Moreover, transgenic plants with reduced expression of Atgrp7 and Atgrp8 will be generated to investigate the phenotypical consequences of reduced expression for morphology and physiology of the plants. To obtain a comprehensive overview about the conditions under which AtGRP7 and AtGRP8 may preferentially be active, their expression pattern during development and in response to external stimuli and stress factors will be investigated in detail in consideration of the underlying clock regulation.
该项目的总体目标是对时钟控制的RNA结合蛋白AtGRP 7(拟南芥富含甘氨酸的RNA结合蛋白)和AtGRP 8进行功能表征。AtGRP 7是负反馈回路的一部分,通过该负反馈回路,它调节自身转录物以及atgrp 8转录物的振荡。我们认为AtGRP 7反馈回路参与了将中央生物钟产生的定时信号传输到细胞内的时钟控制过程。为了定义这个反馈回路的功能,我们建议确定AtGRP 7的目标转录本。由于预期此类转录物在组成型过表达AtGRP 7(AtGRP 7-ox)的转基因植物中显示改变的表达,因此将使用DNA微阵列比较野生型植物和AtGRP 7-ox植物之间的转录物谱。此外,将产生Atgrp 7和Atgrp 8表达降低的转基因植物,以研究表达降低对植物形态和生理的表型后果。为了获得关于AtGRP 7和AtGRP 8可能优先活跃的条件的全面概述,将考虑潜在的时钟调节来详细研究它们在发育期间以及响应于外部刺激和应激因素的表达模式。
项目成果
期刊论文数量(7)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
A glycine-rich RNA-binding protein affects gibberellin biosynthesis in Arabidopsis
- DOI:10.1007/s11033-013-2878-7
- 发表时间:2013
- 期刊:
- 影响因子:2.8
- 作者:B. Löhr;C. Streitner;A. Steffen;T. Lange;D. Staiger
- 通讯作者:B. Löhr;C. Streitner;A. Steffen;T. Lange;D. Staiger
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Professorin Dr. Dorothee Staiger其他文献
Professorin Dr. Dorothee Staiger的其他文献
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{{ truncateString('Professorin Dr. Dorothee Staiger', 18)}}的其他基金
Spatiotemporal analysis of in vivo RNA-protein interaction
体内 RNA-蛋白质相互作用的时空分析
- 批准号:
422321065 - 财政年份:2019
- 资助金额:
-- - 项目类别:
Research Grants
U1 snRNP and polyadenylation in plants – a novel liaison
U1 snRNP 和植物中的聚腺苷酸化——一种新的联系
- 批准号:
426553355 - 财政年份:2019
- 资助金额:
-- - 项目类别:
Research Grants
Single mRNA interactome capture in Arabidopsis - RAPping rhythms
拟南芥中单个 mRNA 相互作用组捕获 - RAPping 节律
- 批准号:
391749644 - 财政年份:2018
- 资助金额:
-- - 项目类别:
Research Grants
Global Impact of Arginine Methylation on Splicing in Arabidopsis
精氨酸甲基化对拟南芥剪接的整体影响
- 批准号:
262541485 - 财政年份:2015
- 资助金额:
-- - 项目类别:
Research Grants
Unravelling the role of an autonomous pathway component in FTi control in Arabidopsis and barley
揭示自主途径成分在拟南芥和大麦 FTi 控制中的作用
- 批准号:
196931130 - 财政年份:2011
- 资助金额:
-- - 项目类别:
Priority Programmes
Mutual interactions between biological timing and innate immunity in Arabidopsis
拟南芥生物计时与先天免疫之间的相互作用
- 批准号:
71247354 - 财政年份:2008
- 资助金额:
-- - 项目类别:
Research Grants
Redox-Regulation der Wechselbeziehung zwischen Photosynthese, Respiration und N-Stoffwechsel in Cyanobakterien
蓝藻光合作用、呼吸和氮代谢之间相互关系的氧化还原调节
- 批准号:
5258598 - 财政年份:2000
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-- - 项目类别:
Research Units
Splicing Factors and riboregulators in the control of thermo-resilience in plants
剪接因子和核糖调节剂在植物耐热性控制中的作用
- 批准号:
505658853 - 财政年份:
- 资助金额:
-- - 项目类别:
Research Grants
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