Efficient generation of functional hepatocytes from human induced pluripotent stem cells and isolation of hepatic progenitor cells

本研究的目的是建立从人诱导多能干细胞（iPSC）产生功能性肝细胞。这表明使用表达人HNF6的腺病毒的肝分化方法有助于从iPSC有效地发育肝细胞。将HNF6导入iPSHC中，CYP3A4表达显著增加，并且细胞具有代谢睾酮的能力。我们的研究首次证明了HNF6在诱导iPSCs分化的肝细胞样细胞中对CYP3A4基因具有反式激活作用。

The purpose of this study was to establish the generation of functional hepatocytes from human induced pluripotent stem cells (iPSCs). It revealed that the hepatic differentiation method using human HNF6-expressing adenovirus contributed to the efficient development of hepatocytes from iPSCs. HNF6 was introduced into the iPSHCs, CYP3A4 expression dramatically increased and the cells had the capability to metabolize testosterone. Our study proved for the first time that HNF6 transactivates the CYP3A4gene in hepatocyte-like cells differentiated from iPSCs.