Microbial diversity and key activities at hydrothermal habitats of the Mid-Atlantic Ridge

大西洋中脊热液栖息地的微生物多样性和主要活动

• 批准号: 5415791
• 负责人: Professor Dr. Johannes F. Imhoff
• 金额: --
• 依托单位: Forschungsbereich 3: Marine Ökologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2003
• 资助国家: 德国
• 起止时间: 2002-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5415791?language=en
• 关键词: Microbial; diversity; key; activities; hydrothermal

Prospering microbial communities developing at hydrothermal vent sites are strictly dependent on the hydrothermal fluid flow. They are primary producers living at the expense of energy available in the reduced substrates provided by the geochemical processes of hydrothermal vents. Knowledge on mircrobial diversity and ecological niches at hydrothermal vent ecosystems is still fragmentary. The proposed work will focus on the diversity of microbial communities associated with hydrothermal vent habitats of the Mid-Atlantic Ridge and function related analysis of these communities. It will include eubacterial and archaeal diversity as well as diversity of functional genes of importance for these ecosystems, which code for CO2 fixation, aerobib methane oxidation (pmoA) and sulfur oxidation (soxB). The distribution of major representative groups of microoranisms along temperature and chemical gradients will be determined, important physiological functions will be measured, and the microorganisms involved in these transformations will be identified. The proposed work will include experimental set ups to investigate temperature adaptation of natural microbial communities within artificial temperature gradients, activity measurements with natural samples, isolation and characterisation of the pure cultures.

在热液喷口处发育的繁荣的微生物群落严格依赖于热液流体流动。它们是初级生产者，依靠热液喷口的地球化学过程提供的还原基质中的能量为生。关于热液喷口生态系统微生物多样性和生态位的知识仍然是零碎的。拟议的工作将侧重于与中大西洋海脊热液喷口生境有关的微生物群落的多样性，以及对这些群落进行与功能有关的分析。它将包括真细菌和古细菌的多样性以及对这些生态系统重要的功能基因的多样性，这些基因编码CO2固定、好氧甲烷氧化（pmoA）和硫氧化（soxB）。将确定微生物沿着温度和化学梯度的主要代表性类群的分布，测量重要的生理功能，并鉴定参与这些转化的微生物。拟议的工作将包括实验设置，以调查人工温度梯度内的天然微生物群落的温度适应性，天然样品的活性测量，纯培养物的分离和表征。