Bone-marrow adipocytes as a new cell source for cell therapy in osteoporosis and aplastic anemia

骨髓脂肪细胞作为骨质疏松症和再生障碍性贫血细胞治疗的新细胞来源

• 批准号: 24790664
• 负责人: KAZAMA tomohiko
• 金额: $ 2.58万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24790664/
• 关键词: DFAT; 脱分化; 骨髄中間葉系幹細胞; 骨粗鬆症; 再生不良性貧血

In BM-DFAT prepared from bone marrow fluid from patients undergoing implant arthroplasty, osteogenic-specific genes were expressed equivalently in BM-MSC prepared from same donor. Alkaline phosphatase was strongly expressed before and during osteogenic differentiation induction, and mineralized matrix aggregates were observed after 2 weeks of differentiation induction by staining for alizarin red S. In a mouse bone fracture model, bone density in BM-DFAT grafts were significantly higher compared with those in control grafts.In BM-DFAT prepared from bone marrow fluid from patients with aplastic anemia, proteins and mRNA levels for SDF-1 were significantly higher than BM-MSC prepared from same donor. Implantation of these BM-DFAT and human umbilical cord blood (UCB) CD34+ cells into a NOD/SCID mouse model of graft failure of human UCB resulted in a detection of human blood cell fractions. These results indicated BM-DFAT possess the promoting effects of engraftment of human UCB.

在从接受植入关节成形术的患者的骨髓液制备的BM-DFAT中，成骨特异性基因在从相同供体制备的BM-MSC中表达相等。碱性磷酸酶在成骨分化诱导前和诱导过程中强烈表达，通过茜素红S染色，在分化诱导2周后观察到矿化基质聚集体。在小鼠骨折模型中，BM-DFAT移植物的骨密度显著高于对照移植物，再生障碍性贫血患者骨髓液制备的BM-DFAT中SDF-1的蛋白和mRNA水平显著高于同一供体的BM-MSC。将这些BM-DFAT和人脐带血（UCB）CD 34+细胞植入人UCB移植失败的NOD/SCID小鼠模型中导致检测到人血细胞级分。这些结果表明BM-DFAT具有促进人脐血移植的作用。

项目成果

骨欠損および骨粗鬆症に対する脱分化脂肪細胞(DFAT)自家移植の効果

去分化脂肪细胞（DFAT）自体移植对骨缺损和骨质疏松的影响

• 发表时间: 2014
• 作者: 風間智彦;菊田晋祐;田中伸明;風間美奈子;徳橋泰明;加野浩一郎;松本太郎
• 通讯作者: 松本太郎

Osteogenic effects of dedifferentiated fat (DFAT) cell transplantation in rabbit models of bone defect and ovariectomy (OVX)-induced osteoporosis.

去分化脂肪（DFAT）细胞移植对骨缺损和卵巢切除（OVX）诱导的骨质疏松兔模型的成骨作用。

• 发表时间: 2013
• 期刊: Tissue Engineering
• 作者: Kikuta S;Tanaka N;Kazama T;Kazama M;Kano K;Ryu J;Tokuhashi Y;Matsumoto T
• 通讯作者: Matsumoto T

Phenotypic and functional properties of feline dedifferentiated fat cells and adipose-derived stem cells

• DOI: 10.1016/j.tvjl.2013.10.033
• 发表时间: 2014-01-01
• 期刊: VETERINARY JOURNAL
• 影响因子: 2.2
• 作者: Kono, Shota;Kazama, Tomohiko;Matsumoto, Taro
• 通讯作者: Matsumoto, Taro

骨欠損および骨粗鬆症に対する脱分化脂肪細胞（DFAT）自家移植の効果

去分化脂肪细胞（DFAT）自体移植物对骨缺损和骨质疏松症的影响

• 发表时间: 2014
• 作者: 風間智彦;菊田晋祐;田中伸明;風間美奈子;徳橋泰明;加野浩一郎;松本太郎
• 通讯作者: 松本太郎