Bone-marrow adipocytes as a new cell source for cell therapy in osteoporosis and aplastic anemia

在由接受人工关节置换术的患者骨髓液制备的BM-DFAT中，成骨特异性基因在同一供体制备的BM-MSC中表达相同。碱性磷酸酶在成骨分化诱导前和诱导过程中均有强烈表达，诱导2周后茜素红s染色观察到矿化基质聚集体。在小鼠骨折模型中，BM-DFAT移植物的骨密度明显高于对照移植物。再生障碍性贫血患者骨髓液制备的BM-DFAT中，SDF-1蛋白和mRNA水平显著高于同一供者制备的BM-MSC。将这些BM-DFAT和人脐带血（UCB） CD34+细胞植入NOD/SCID人脐带血（UCB）移植失败小鼠模型中，检测到人血细胞部分。这些结果表明BM-DFAT具有促进人UCB移植的作用。

In BM-DFAT prepared from bone marrow fluid from patients undergoing implant arthroplasty, osteogenic-specific genes were expressed equivalently in BM-MSC prepared from same donor. Alkaline phosphatase was strongly expressed before and during osteogenic differentiation induction, and mineralized matrix aggregates were observed after 2 weeks of differentiation induction by staining for alizarin red S. In a mouse bone fracture model, bone density in BM-DFAT grafts were significantly higher compared with those in control grafts.In BM-DFAT prepared from bone marrow fluid from patients with aplastic anemia, proteins and mRNA levels for SDF-1 were significantly higher than BM-MSC prepared from same donor. Implantation of these BM-DFAT and human umbilical cord blood (UCB) CD34+ cells into a NOD/SCID mouse model of graft failure of human UCB resulted in a detection of human blood cell fractions. These results indicated BM-DFAT possess the promoting effects of engraftment of human UCB.