Identification of human hematopoietic stem cell regulators with HLA allele deficient cells behavior

鉴定具有 HLA 等位基因缺陷细胞行为的人类造血干细胞调节因子

• 批准号: 24790968
• 负责人: KATAGIRI Takamasa
• 金额: $ 2.75万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24790968/
• 关键词: Loss of heterogeneity; Aplastic anemia; Human Leukocyte Antigen; 病態検査学; 血液内科学; 免疫学; 再生不良性貧血; uniparental disomy; HLA

The percentage of 6pLOH was different among hematopoiesis stages in human bone marrow, and the progenitor cells showed much lower percentage of 6pLOH than that of MEP and GMP.I found approximately 30 times lower expression of CXCL12 which promotes the cell growth and proliferation cytokine than that of normal fraction with microarray analysis. As a result, I demonstrated the percentage of HLA deficient was significantly decreased, since the cell cycle was not promoted in the 6pLOH of CMP fraction with low CXCL12 and CXCR4 expression.

人骨髓造血不同阶段6pLOH的比例不同，6pLOH的比例在造血祖细胞中明显低于MEP和GMP，而促进细胞生长和增殖的细胞因子CXCL 12的表达比正常组份低约30倍。结果，我证明HLA缺陷的百分比显著降低，因为在具有低CXCL 12和CXCR 4表达的CMP组分的6pLOH中，细胞周期没有被促进。

项目成果

Increased Gene Expression Of the Nr4A Family In Bone Marrow T Cells Of Patients With Early Stage Acquired Aplastic Anemia

早期获得性再生障碍性贫血患者骨髓 T 细胞中 Nr4A 家族基因表达增加

• 发表时间: 2013
• 作者: 丸山 裕之;佐治 博夫;屋部登志雄;柏瀬 貢一;片桐 孝和;材木 義隆;斉藤 千鶴;細川 晃平;山崎 宏人;小川 誠司;中尾 眞二;Takamasa Katagiri;Kohei Hosokawa
• 通讯作者: Kohei Hosokawa

Prevalence of HLA-A allele lacking leukocytes in patients with aplastic anemia at diagnosis

再生障碍性贫血患者诊断时缺乏白细胞的 HLA-A 等位基因的患病率

• 发表时间: 2013
• 作者: 丸山 裕之;佐治 博夫;屋部登志雄;柏瀬 貢一;片桐 孝和;材木 義隆;斉藤 千鶴;細川 晃平;山崎 宏人;小川 誠司;中尾 眞二;Takamasa Katagiri;Kohei Hosokawa;Hiroyuki Maruyama;Tetsuichi Yoshizato;Takamasa Katagiri;Yoshiki Akatsuka;Chizuru Saito;Tetsuichi Yoshizato;Hiroyuki Maruyama
• 通讯作者: Hiroyuki Maruyama

Immunologically escaped hematopoiesis caused by HLA allelic loss in patients with aplastic anemia

再生障碍性贫血患者 HLA 等位基因缺失导致免疫逃逸造血

• 发表时间: 2012
• 期刊: Rinsho Ketsueki
• 作者: Takamasa Katagiri;Shinji Nakao
• 通讯作者: Shinji Nakao

Evidence That GPI-AP-Specific CTLs Are Not Involved In The “escape” Of Piga mutant Hematopoietic Stem Cells In Aplastic Anemia

证据表明 GPI-AP 特异性 CTL 不参与再生障碍性贫血中 Piga 突变造血干细胞的“逃逸”

• 发表时间: 2013
• 作者: Takamasa Katagiri;Hiroyuki Maruyama;Shigeki Ohtake;Chizuru Saito;Kohei Hosokawa;Seishi Ogawa;and Shinji Nakao
• 通讯作者: and Shinji Nakao

Escape hematopoiesis by HSC with somatic SLIT1 mutaions in patients with acquired aplastic anemia

获得性再生障碍性贫血患者体细胞 SLIT1 突变的 HSC 逃逸造血

• 发表时间: 2013
• 作者: Chizuru Saito;Kohei Hosokawa;Hiroyuki Maruyama;Takamasa Katagiri;Akinori Kanai;Hirotaka Matsui;Toshiya Inaba;Akari Kazami;Masafumi Taniwaki;Hirohito Yamazaki;Shinji Nakao
• 通讯作者: Shinji Nakao