Autophagy regulates galectin-4 expression in the rat trophoblast cell line and affects its differentaiton

本研究旨在探讨Gal4在胎盘中的表达。我们使用了来自大鼠胎盘的Rcho-1细胞，该细胞系已被很好地建立为滋养细胞分化的模型系统。在分化的Rcho-1细胞中Gal4的表达低于增殖细胞。有趣的是，在Rcho-1分化过程中，Gal4的过表达导致细胞流动性和细胞增大的适度降低。值得注意的是，免疫组织化学分析显示Gal4优先位于大鼠胎盘母胎区，这表明Gal4会负向调节Rcho-1细胞的分化和/或限制其侵袭性。

This study aimed to investigate the expression of Gal4 in placentation. We utilized Rcho-1 cells derived from rat placenta and this cell line has been well established as a model system for trophoblast differentiation. The expression of Gal4 was revealed to be lower in differentiated Rcho-1 cells than that in proliferative cells. Of interest, overexpression of Gal4 during Rcho-1 differentiation resulted in moderate reduction of both cell mobility and cell enlargement. Notably, immunohistochemical analysis showed that Gal4 is preferentially located in the maternal-fetal zone of rat placenta, indicating that Gal4 would negatively regulate differentiation of Rcho-1 cells and/or limit their invasive property.