Establishment of novel culturing system for salivary gland cells
新型唾液腺细胞培养体系的建立
基本信息
- 批准号:24792131
- 负责人:
- 金额:$ 2.66万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Young Scientists (B)
- 财政年份:2012
- 资助国家:日本
- 起止时间:2012-04-01 至 2014-03-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Salivary gland (SG) dysfunction occurs as a result of radiation therapy for head cancer, and can cause a variety of critical oral health issues. In the present study, we demonstrated here a new culture system using rock-related agent (RRA) for SG cells. In addition to protein expression data that downstream molecules of RRA treatment associated with morphological changes were inhibited, we found that the RRA-treated cells showed SG like cell shape without fibroblastic differentiation, and their zenografts as well as the RRA-treated cells highly expressed the SG markers (amylase, aquaporin-5, and mucin). Furthermore, we assessed cytotoxicity of RRA to see whether we can use those reagents for clinical study. Karyotype and toxicity tests showed that RRA does not affect any genetic changes and pathological damages in vitro and in vivo models. These data suggest that our method would be important information for cell therapy of damaged SG in the near future.
唾液腺(SG)功能障碍是由于头部癌症的放射治疗而发生的,并可能导致各种严重的口腔健康问题。在本研究中,我们展示了一种新的培养系统,使用岩石相关剂(RRA)的SG细胞。除了与形态学变化相关的RRA处理的下游分子被抑制的蛋白质表达数据之外,我们发现RRA处理的细胞显示SG样细胞形状而没有成纤维细胞分化,并且它们的异种移植物以及RRA处理的细胞高度表达SG标记物(淀粉酶、水通道蛋白-5和粘蛋白)。此外,我们还评估了RRA的细胞毒性,以确定是否可以将这些试剂用于临床研究。核型和毒性试验表明,RRA在体外和体内模型中不影响任何遗传改变和病理损伤。这些数据表明,我们的方法将是重要的信息,在不久的将来受损SG的细胞治疗。
项目成果
期刊论文数量(0)
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KASAMATSU Atsushi其他文献
KASAMATSU Atsushi的其他文献
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{{ truncateString('KASAMATSU Atsushi', 18)}}的其他基金
Establishment of long-term stability culturing system for salivary gland cells
唾液腺细胞长期稳定培养体系的建立
- 批准号:
26861668 - 财政年份:2014
- 资助金额:
$ 2.66万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Roles of intraoral digestion on regulation of glucose metabolism
口腔内消化对葡萄糖代谢调节的作用
- 批准号:
21890040 - 财政年份:2009
- 资助金额:
$ 2.66万 - 项目类别:
Grant-in-Aid for Research Activity Start-up