Development of gene therapy for oral cancer mediated by Naked DNA of molecule processing E-cadherin

分子加工E-钙粘蛋白的裸DNA介导的口腔癌基因治疗的进展

• 批准号: 24792218
• 负责人: HAMANA Tomoaki
• 金额: $ 2.75万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24792218/
• 关键词: α２－アンチプラスミン; プラスミン; E-カドヘリン; Naked DNA; 口腔癌遺伝子治療; α2ーアンチプラスミン; Eーカドヘリン; Eｰカドヘリン; α2ｰアンチプラスミン

This study showed that oral cancer cells transferred gene of alpha2-antiplasmin (alpha2-AP) which is plasmin inhibitor were remarkably suppressed in vivo tumor formation and expression of E-cadherin increased in the formed tumor. The results suggest that inducing of alpha2-AP protein suppresses tumor invasion and metastasis by increasing not only proteolytic activity but also cell migration and proliferation. Therefor, it is expected the development of novel gene therapy by inducing of alpha2-AP for oral cancer.

本研究表明，转导纤溶酶抑制物α2-抗纤溶酶基因的口腔癌细胞体内肿瘤形成受到明显抑制，E-钙粘附素表达增强。结果表明，α2-AP蛋白的诱导不仅通过增加蛋白水解酶的活性，而且通过增加细胞的迁移和增殖来抑制肿瘤的侵袭和转移。因此，通过诱导α2-AP基因治疗口腔癌有望开发新的基因治疗方法。