Physicochemical studies of YB-1 functions and structures

Y-box binding protein 1（YB-1）是一种调节转录和翻译的核酸结合蛋白，在癌细胞中过表达。据报道，YB-1与特定基因组dna或mrna的结合参与了肿瘤的发生。因此，我们有理由认为，由于治疗人类疾病和阐明新的细胞功能，YB-1是调节细胞系统的新靶点。然而，YB-1与核酸的结合机制尚不清楚。本文采用分光光度法和量热法对YB-1的结构和结合特性进行了理化分析。重组蛋白YB-1与含有Y-box序列的单链DNA和含有Y-box mRNA序列的单链DNA具有独特的热力学结合。利用YB-1全长突变体和缺失突变体鉴定了YB-1的DNA和RNA结合位点。我们进一步研究了盐和温度对其与DNA或RNA结合的影响。

Y-box binding protein 1(YB-1) is a nucleic acid-binding protein that regulates transcription and translation, and is overexpressed in cancer cells. It has been reported that the bindings of YB-1 to specific genomic DNAs or mRNAs are involved in tumorigenesis. Therefore, it is plausible to suppose that YB-1 is a new target to regulate the cellular systems due to treatment of human diseases and elucidation of new cellular functions. However, the binding mechanism of YB-1 to nucleic acids is still unclear. Here, we carried out physicochemical analyses of the structural and binding properties of YB-1 using spectrometry and calorimetry. The recombinant YB-1 had thermodynamically unique bindings to the single-strand DNA with Y-box sequence and to the single-strand DNA with Y-box mRNA sequence. The DNA and RNA binding sites of YB-1 were identified by using a full length and deletion mutants of YB-1. We further studied the effects of salt and temperature on the binding to DNA or RNA.