Development of a novel molecular combing method for high throughput analysis of giant DNA molecule

分子梳是一种强大的方法，用于排列巨大的DNA分子，并在从分子生物学到纳米电子学的广泛应用中使用。本研究的目的是发展一种新的高通量分析大DNA分子的分子梳理方法。传统的分子梳理是基于在空气/水界面处施加的力。这项研究提出了利用油/水界面拉伸和折叠巨大的DNA分子。因此，通过将油/水界面和微流体通道相结合，实现了所提出的分子组合。这种基于新概念的组合方法有望成为基因组DNA高通量分析的新工具。

Molecular combing is a powerful method for aligning giant DNA molecules and used in a wide range of applications from molecular biology to nanoelectronics. The purpose of this study is development of a novel molecular combing method for high throughput analysis of giant DNA molecules. The conventional molecular combing is based on the force applied at the air/water interface. This study proposes to use the oil/water interface to stretch and immobilize giant DNA molecules. As a result, by combining the oil/water interface and a microfluidic channel, the proposing molecular combing was achieved. This combing method based on the novel concept is expected to be a new tool in high throughput analysis of genomic DNA..