Development of a novel molecular combing method for high throughput analysis of giant DNA molecule

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Molecular combing is a powerful method for aligning giant DNA molecules and used in a wide range of applications from molecular biology to nanoelectronics. The purpose of this study is development of a novel molecular combing method for high throughput analysis of giant DNA molecules. The conventional molecular combing is based on the force applied at the air/water interface. This study proposes to use the oil/water interface to stretch and immobilize giant DNA molecules. As a result, by combining the oil/water interface and a microfluidic channel, the proposing molecular combing was achieved. This combing method based on the novel concept is expected to be a new tool in high throughput analysis of genomic DNA..