Water-in-oil droplet formation by electrostatic atomization and its application to biotechnology

研究了静电雾化作用下油包水（W/O）液滴的形成和静电力对单个液滴的操纵。研究了一种利用静电场产生W/O液滴的方法。该方法可以通过调节施加的直流高压来控制水滴的大小，并且由于电荷的作用，这些水滴在没有表面活性剂的情况下是稳定的。此外，还研究了一种利用非均匀静电场传输和熔合液滴的新方法。此外，还利用直流电场进行了基因转化。与传统方法相比，该方法可减少样品体积。总之，这些方法有望成为小型化生物工程的新工具。

Water-in-oil(W/O) droplet formation by electrostatic atomization and manipulation of the individual droplet by electrostatic force were studied. A method to generate W/O droplets using electrostatic field was investigated. The method can control the size of water droplets by adjusting the applied DC high voltage and these water droplets are stable without a surfactant due to electrical charge. In addition, a novel method to transport and fuse W/O droplets using non-uniform electrostatic field was also investigated. Furthermore, gene transformation by using DC electric field was carried out. This method can reduce sample volume smaller than that of conventional method. In conclusion, these methods are expected to be a new tool in miniaturized bioengineering.