Water-in-oil droplet formation by electrostatic atomization and its application to biotechnology

静电雾化形成油包水液滴及其在生物技术中的应用

• 批准号: 22760207
• 负责人: KURITA Hirofumi
• 金额: $ 2.58万
• 依托单位: Toyohashi University of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22760207/
• 关键词: 静電噴霧; 油中液滴; バイオ応用; マイクロリアクター; 静電気生物応用; 液滴生成; 遺伝子導入

Water-in-oil(W/O) droplet formation by electrostatic atomization and manipulation of the individual droplet by electrostatic force were studied. A method to generate W/O droplets using electrostatic field was investigated. The method can control the size of water droplets by adjusting the applied DC high voltage and these water droplets are stable without a surfactant due to electrical charge. In addition, a novel method to transport and fuse W/O droplets using non-uniform electrostatic field was also investigated. Furthermore, gene transformation by using DC electric field was carried out. This method can reduce sample volume smaller than that of conventional method. In conclusion, these methods are expected to be a new tool in miniaturized bioengineering.

研究了油包水（W/O）液滴的静电雾化和静电力对液滴的控制。研究了一种利用静电场产生W/O液滴的方法。该方法可以通过调节所施加的DC高压来控制水滴的尺寸，并且这些水滴由于电荷而在没有表面活性剂的情况下是稳定的。此外，本文还研究了一种利用非均匀静电场实现W/O液滴输运和融合的新方法。此外，通过使用直流电场进行基因转化。与传统方法相比，该方法可减少样品体积。总之，这些方法有望成为小型化生物工程的新工具。

项目成果

Generation of Water Droplet in Fluorocarbon using Electrostatic Atomization

利用静电雾化在氟碳化合物中产生水滴

• 发表时间: 2010
• 作者: Hironori Aoki;Hirofumi Kurita;Thierry Paillat;Kazunori Takashima;and Akira Mizuno
• 通讯作者: and Akira Mizuno

不平等電界を用いた油中液滴輸送・融合技術の開発

不等电场油滴输运与聚变技术研究进展

• 发表时间: 2010
• 作者: Hironori Aoki;Hirofumi Kurita;Thierry Paillat;Kazunori Takashima;and Akira Mizuno;青木博則,栗田弘史,安田八郎,高島和則,水野彰
• 通讯作者: 青木博則,栗田弘史,安田八郎,高島和則,水野彰

A kinetic analysis of strand breaks on large DNA induced by cigarette smoke extract

• DOI: 10.1016/j.cplett.2010.05.028
• 发表时间: 2010-06-17
• 期刊: CHEMICAL PHYSICS LETTERS
• 影响因子: 2.8
• 作者: Kurita, Hirofumi;Takata, Tatsuya;Mizuno, Akira
• 通讯作者: Mizuno, Akira

Single-molecule measurement of strand breaks on large DNA induced by atmospheric pressure plasma jet

• DOI: 10.1063/1.3660581
• 发表时间: 2011-11
• 期刊: Applied Physics Letters
• 影响因子: 4
• 作者: H. Kurita;T. Nakajima;H. Yasuda;K. Takashima;A. Mizuno;John I. B. Wilson;S. Cunningham

Water-in-oil droplet generation and manipulation using an electrostatic field for miniaturized reaction systems

使用静电场生成和操纵小型反应系统的油包水液滴

• 发表时间: 2011
• 作者: Atsushi Asada;Hironori Aoki;Hirofumi Kurita;Hachiro Yasuda;Kazunori Takashima;and Akira Mizuno
• 通讯作者: and Akira Mizuno