Development of culture method for evaluation of contractile capability of human skeletal muscle cells

为了提高从人骨骼肌成肌细胞群体中提取的人骨骼肌肌管的主动张力能力，研究了在成肌细胞群体中添加人骨骼肌成纤维细胞的方法。与我们的预期相反，人类骨骼肌肌管的收缩能力并没有得到明显的改善，这可能是由于成纤维细胞在成肌组织中的迁移行为和细胞-细胞连接行为的差异导致成纤维细胞在成肌组织中的空间居住所致。成纤维细胞混合在成肌细胞组织中，呈垂直快速线性迁移，呈上位分布。进一步研究不同类型细胞的居住模式，将有助于控制异质细胞群体的空间排列和构建复杂的组织。

To enhance active tension capability of human skeletal muscle myotubes derived from human skeletal muscle myoblast population, addition of human skeletal muscle fibroblast cells to myoblast population was examined. Contrary to our expectation, significant improvement of contractile capability of human skeletal muscle myotubes was not achieved, which may be caused by spatial habitation of fibroblast cells in the myoblast tissue due to the differences in its behavior of migration and cell-cell connection. Fibroblast cells mixed in the myoblast tissue rapidly and linearly migrated in the vertical direction and distributed in upper position. Further studies investigating the habitation pattern of various cell types will shed light on controlling the spatial arrangement of the heterogeneous cell population and constructing complicated tissues.