Dual, realtime minitoring of Ca2+ and ROS signals in plant cells

植物细胞中 Ca2 和 ROS 信号的双重实时监测

• 批准号: 24770041
• 负责人: FURUICHI Takuya
• 金额: $ 3万
• 依托单位: Gifu Women's University (2013)Nagoya University (2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24770041/
• 关键词: 細胞内シグナル伝達; 活性酸素種; ROS; 生物発光; リアルタイム計測; カルシウムシグナル; ROSシグナル

Reactive oxygen species (ROS) play key roles in intra- / inter-cellular signal transduction. Recent studies revealed that Ca2+-signal and ROS-signal are interplaying for the precise intracellular signal transduction through generating [Ca2+]c oscillation and playing the other key roles of still unknown. In the present study, we tried to develop a novel method to monitor [ROS]c and [Ca2+]c simultaneously using Ca2+- or ROS-sensitive photoproteins and spectrometry of luminescence to clarify the physiological roles and mechanisms of ROS-signal and their interplay with Ca2+-signal in plants.

活性氧（ROS）在细胞内/细胞间信号转导中发挥关键作用。最近的研究表明，Ca2+信号和ROS信号相互作用，通过产生[Ca2+]c振荡来实现精确的细胞内信号转导，并发挥其他尚不清楚的关键作用。在本研究中，我们试图开发一种使用Ca2+或ROS敏感光蛋白和发光光谱法同时监测[ROS]c和[Ca2+]c的新方法，以阐明植物中ROS信号的生理作用和机制及其与Ca2+信号的相互作用。

项目成果

Real-time monitoring of the intracellular ROS signal using a novel photoprotein

使用新型发光蛋白实时监测细胞内 ROS 信号

• 发表时间: 2012
• 作者: Furuichi;T
• 通讯作者: T

Calcium Mobilizations in Response to Changes in the Gravity Vector in Arabidopsis Seedlings : Possible Cellular Mechanisms

拟南芥幼苗响应重力矢量变化的钙动员：可能的细胞机制

• 发表时间: 2014
• 期刊: Plant Signal. Behav.
• 作者: Tatsumi;H.;Toyota;M.;Furuichi;T.;and Sokabe;M.
• 通讯作者: M.

Mechanosensitive channels are activated via actin stress fibers, which may be involved in gravity sensing in plants

机械敏感通道通过肌动蛋白应力纤维激活，可能参与植物的重力感应

• 发表时间: 2012
• 作者: Hitoshi Tatsumi;Takuya Furuichi;Masataka Nakano;Masatsugu Toyota;Kimihide Hayakawa;Masahiro Sokabe;and Hidetoshi Iida
• 通讯作者: and Hidetoshi Iida

Roles and mechanisms of calcium signaling in plant environmental responses

钙信号在植物环境反应中的作用和机制

• 发表时间: 2012
• 作者: Furuichi;T
• 通讯作者: T

Analyses of a Gravistimulation-Specific Ca2+ Signature in Arabidopsis using Parabolic Flights

• DOI: 10.1104/pp.113.223313
• 发表时间: 2013-10-01
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Toyota, Masatsugu;Furuichi, Takuya;Tatsumi, Hitoshi
• 通讯作者: Tatsumi, Hitoshi