Molecular mechanisms of wound-triggered cell dedifferentiation

伤口触发细胞去分化的分子机制

• 批准号: 24770053
• 负责人: IWASE AKIRA
• 金额: $ 3万
• 依托单位: Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24770053/
• 关键词: 脱分化; 分化全能性; カルス形成; ストレス応答; AP2/ERF型転写因子; 細胞リプログラミング; 植物ホルモン; リプログラミング; カルス; 転写因子; 再分化; 傷害; ストレス; 活性酸素

WIND1 is wound inducible transcription factor which positively regulates cell dedifferentiation and callus formation at wound site of Arabidopsis. Yeast one hybrid analysis revealed that a heat shock (HS)-related transcription factor is one candidates of WIND1-upstream factor. This factor is upregulated by not only heat shock but also by wound. When plants are treated on heat-stress condition, WIND1 expression is upregulated and plant comes to form callus easily. WIND1 loss of function suppresses this callus induction, suggesting WIND1 participates in heat inducible cell dedifferentiation. Level of WIND1 expression in the loss-of-function mutant of the HS-related transcription factor is smaller than that of wild-type plants under both normal and heat stress condition. These results and other observations clarified that there is HS-triggered cell dedifferentiation cascade, which is mediated by HS-related factor and WIND1.

WIND1是一种创伤诱导转录因子，它能正向调节拟南芥创伤部位的细胞脱分化和愈伤组织的形成。酵母单杂交分析表明，一个与热休克(HS)相关的转录因子是WIND1-上游因子的候选因子之一。这一因子不仅因热休克而上调，也因创伤而上调。植物在高温胁迫条件下，WIND1表达上调，容易形成愈伤组织。WIND1功能的丧失抑制了这种愈伤组织的诱导，表明WIND1参与了热诱导细胞的去分化。在正常和热胁迫条件下，HS相关转录因子功能缺失突变体的WIND1表达水平均低于野生型植物。这些结果和其他观察结果表明，存在HS触发的细胞去分化级联反应，这是由HS相关因子和WIND1介导的。

项目成果

Molecular network of plant dedifferentiation

植物去分化的分子网络

• 发表时间: 2012
• 作者: Akira Iwase;Momoko Ikeuchi;Mariko Ohnuma;Keiko Sugimoto
• 通讯作者: Keiko Sugimoto

Molecular network of plant cell dedifferentiation

植物细胞去分化的分子网络

• 发表时间: 2014
• 作者: Akira Iwase;Momoko Ikeuchi;Mariko Ohnuma;Keiko Sugimoto
• 通讯作者: Keiko Sugimoto

The RNA-binding protein FPA regulates flg22-triggered defense responses and transcription factor activity by alternative polyadenylation.

• DOI: 10.1038/srep02866
• 发表时间: 2013-10-09
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Lyons, Rebecca;Iwase, Akira;Gansewig, Thomas;Sherstnev, Alexander;Duc, Celine;Barton, Geoffrey J.;Hanada, Kousuke;Higuchi-Takeuchi, Mieko;Matsui, Minami;Sugimoto, Keiko;Kazan, Kemal;Simpson, Gordon G.;Shirasu, Ken
• 通讯作者: Shirasu, Ken

Plant cell dedifferentiation under environmental stress

环境胁迫下植物细胞去分化

• 发表时间: 2013
• 作者: Akira Iwase;Momoko Ikeuchi;Mariko Ohnuma;Keiko Sugimoto
• 通讯作者: Keiko Sugimoto

The AP2/ERF Transcription Factor WIND1 Controls Cell Dedifferentiation in Arabidopsis

• DOI: 10.1016/j.cub.2011.02.020
• 发表时间: 2011-03-22
• 期刊: CURRENT BIOLOGY
• 影响因子: 9.2
• 作者: Iwase, Akira;Mitsuda, Nobutaka;Ohme-Takagi, Masaru
• 通讯作者: Ohme-Takagi, Masaru