Epigenetic regulation of key developmental genes from embryo to adult

从胚胎到成体关键发育基因的表观遗传调控

• 批准号: 24770202
• 负责人: TSUKAHARA Tatsuya
• 金额: $ 2.91万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24770202/
• 关键词: エピジェネティクス; 発生関連遺伝子; DNAメチル化; メダカ; 脊椎動物; developmental gene; key developmental gene; ヒストン; 未分化状態

We performed the epigenomic analysis of pluripotent blastula embryos and adult myotome and liver in medaka fish. We revealed that, in developmental gene loci, the size of hypomethylated DNA domain (HMD) positively correlates with the repression levels of developmental gene expression in blastula, and HMDs become shortened when the adjacent gene is actively expressed in adult tissues. The comparative analyses with human ES cells, zebrafish embryos and adult muscle showed the conservation of the correlation between HMD size and the repression levels of developmental genes. Therefore, this study highlights the novel features of developmental gene regulation mediated by the size of epigenetic domain from pluripotent embryos to adult.

我们进行了青鳉鱼多能囊胚胚胎和成体肌节和肝脏的表观基因组分析。 我们发现，在发育基因位点中，低甲基化DNA结构域（HMD）的大小与囊胚发育基因表达的抑制水平呈正相关，当邻近基因在成体组织中活跃表达时，HMD会缩短。与人ES细胞、斑马鱼胚胎和成体肌肉的比较分析表明，HMD大小与发育基因抑制水平之间的相关性是保守的。因此，本研究突出了从多能胚胎到成体由表观遗传结构域大小介导的发育基因调控的新特征。

项目成果

The size of DNA hypomethylated domain reflects repression levels of developmental genes

DNA低甲基化结构域的大小反映了发育基因的抑制水平

• 发表时间: 2013
• 作者: Ryohei Nakamura;Tatsuya Tsukahara;Hiroyuki Takeda
• 通讯作者: Hiroyuki Takeda

Antagonistic relationship between H3K27me3 and DNA methylation in long-term regulation of developmental genes

H3K27me3与DNA甲基化在发育基因长期调控中的拮抗关系

• 发表时间: 2013
• 作者: Miriam Lopez-de-la-Calleja;Takayuki Nagai;Muhammad Attamimi;Mariko Nakano-Miyatake;Hector Perez-Meana;安東裕司，アッタミミ ムハンマド，中村友昭，長井隆行，持橋大地，小林一郎，麻生英樹;中村遼平;山口拓郎，アッタミミ ムハンマド，中村友昭，長井隆行，池原雅章;中村遼平
• 通讯作者: 中村遼平

Large hypomethylated domain serves as strong repressive machinery for key developmental genes in vertebrates

大的低甲基化结构域是脊椎动物关键发育基因的强大抑制机制

• 发表时间: 2014
• 期刊: Development
• 影响因子: 4.6
• 作者: Ryohei Nakamura;Tatsuya Tsukahara;Wei Qu;Kazuki Ichikawa;Takayoshi Otsuka;Katsumi Ogoshi;Taro L Saito;Kouji Matsushima;Sumio Sugano;Shinichi Hashimoto;Yutaka Suzuki;Shinichi Morishita;and Hiroyuki Takeda.
• 通讯作者: and Hiroyuki Takeda.

Epigenetic Regulation of Key Developmental Genes in the Medaka Genome

青鳉基因组中关键发育基因的表观遗传调控

• 发表时间: 2013
• 作者: Ryohei Nakamura;Tatsuya Tsukahara;Hiroyuki Takeda;Tatsuya Tsukahara
• 通讯作者: Tatsuya Tsukahara