Development of in vivo DNA damage/gene mutation assays using DNA adductome analysis

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The applicability of the DNA adductome analysis using LC-MS/MS was demonstrated by the detection of various specific DNA adducts in the livers or kidneys of rats given estragole or rubiadin. In addition, application of DNA adductome analysis to reporter gene mutation assay demonstrated several specific-DNA adducts formation and gene mutation accompanied by characteristic A:T-T:A transversion in the kidneys and livers of gpt delta mice given aristolochic acid, a renal carcinogen. These data suggest that application of DNA adductome analysis to gpt delta animals can be a powerful tool for investigating the genotoxicity.