Interaction analysis of the blood-brain barrier (BBB) constitution cell in the experimental cerebral ischemia model

血脑屏障(BBB)是神经血管单位(NVU)的一员，在脑毛细血管内皮细胞与其他邻近细胞如星形胶质细胞和周细胞之间起通讯作用。血脑屏障功能障碍在脑水肿形成和中枢神经系统紊乱中起着重要作用。因此，病理条件下的实验性血脑屏障功能障碍模型适用于寻找针对血脑屏障功能障碍的保护剂。我们利用大鼠脑内三种主要的血脑屏障相关细胞的原代培养，建立并表征了一种新的体外血脑屏障模型。我们尝试制作了三种BBB功能障碍模型，并成功地制作了如下模型：(1)缺氧/复氧模型，(2)高血糖模型，(3)年龄相关性缺血模型。我们发现，西洛他唑通过抑制转化生长因子-β信号通路，保护血脑屏障免受年龄相关性缺血的影响。

The blood-brain barrier (BBB), a member of the neurovascular unit (NVU), functions in communication between brain capillary endothelium and other neighboring cells such as astrocytes and pericytes. BBB dysfunction plays a crucial role in brain edema formation and central nervous system disorders. Therefore, the experimental BBB dysfunction models under the pathological condition are applicable to search the protective agents against BBB dysfunction.We have developed and characterized a new in vitro BBB model (BBB Kit) using primary cultures of the three main BBB-related cells obtained from rat brains. We attempted to make three types of BBB dysfunction models and succeeded in making following models: (i) hypoxia/reoxygenation model, (ii) hyperglycemia model, (iii) AGE-related ischemia model. We found that cilostazol protected the BBB against AGE-related ischemia, through an inhibitory mechanism on TGF-beta signaling.