Spatio-temporal crosstalk between Ly49Q-mediated immune response and autophagy

为了研究炎症免疫细胞上表达的抑制受体Ly49Q与自噬通路之间的关系，我们使用Ly49Q基因敲除小鼠的炎症免疫细胞和Ly49Q高表达或低表达的RAW264.7细胞系分析细胞反应。通过对参与自噬的组织蛋白酶成熟、炎症刺激下细胞因子表达谱、炎症刺激后内体/溶酶体细胞器定位变化的分析，认为Ly49Q参与了自噬抑制途径和炎症免疫细胞内细胞器运输的调控。

To examine the relationship between Ly49Q, an inhibitory receptor expressed on inflammatory immune cells, and autophagy pathway which plays a significant roles for homeostasis, cellular response was analyzed using inflammatory immune cells from Ly49Q-knockout mice and RAW264.7 cell lines in which Ly49Q expression was high or low. On the basis of analyses of cathepsin-maturation involving in the autophagy, cytokine expression profiles in response to inflammatory stimuli, localization changes of endosomal/lysosomal organelles after inflammatory stimuli, it was suggested that Ly49Q involves in the inhibitory pathway for autophagy and the regulation of organelle-trafficking in inflammatory immune cells.