Physiological function of Integrin in mechanical response of bone

成骨细胞系细胞，尤其是骨细胞，被认为是骨中的主要力学感觉细胞。然而，机械受体和下游机械信号通路的身份仍然在很大程度上未知。使用原代成骨细胞机械流体剪切应力刺激，涉及c-Src，p130 Cas和JNK的细胞内激酶级联被确定为整合素α-V的下游。小鼠缺乏整合素α-V的成骨细胞系表现出受损的骨骼反应尺骨负荷，在Sost表达方面，指向整合素α-V在体内机械感应的作用。因此，整合素α-v可能是成骨细胞谱系细胞中机械感应机制的组成部分，并参与响应机械刺激的Src-JNK-雅普/TAZ途径的激活。

Osteoblast lineage cells, especially osteocytes, are thought to be a primary mechanosensory cell in bone. However, the identity of the mechano-receptor and downstream mechno signaling pathways remain largely unknown. Using primary osteoblastic cells mechanically stimulated with fluid shear stress, an intracellular kinase cascade involving c-Src, p130Cas and JNK was identified the downstream of integrin alpha-v. Mice deficient in integrin alpha-v in the osteoblast lineage exhibited an impaired skeletal response to ulnar loading, in terms of Sost expression, pointing to the role of integrin alpha-v in mechano-sensing in vivo. Thus, integirn alpha-v may be integral to a mechanosensing machinery in osteoblast lineage cells and involved in the activation of a Src-JNK-YAP/TAZ pathway in response to mechanical stimulation.