Identification of bacterial factors that determine the host specificity of bordetellosis.

确定决定博氏菌病宿主特异性的细菌因素。

• 批准号: 24790415
• 负责人: FUKUI Aya
• 金额: $ 2.83万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24790415/
• 关键词: 百日咳菌; 気管支敗血症菌; 宿主特異性

The aim of this project was to identify bacterial factors that determine the host specificity of bordetellosis through analyses of the bacterial gene expression during infection by IVET-IP. I established a rat model of bordetella infection, and found that B. pertussis did not colonize rats, suggesting that the original plan of this project would be unsuccessful. Therefore, tried was an alternative plan, in which B. pertussis derivatives that colonize rats are generated by introduction of genes of B. bronchiseptica that shows a wide host range. I expect that genes conferring the wide host range of B. bronchiseptica and determining the host specificity could be identified by this strategy.

本项目的目的是通过IVET-IP感染过程中细菌基因表达的分析，确定决定博德特氏菌病宿主特异性的细菌因素。建立了大鼠博德特氏菌感染模型，发现B。百日咳没有在老鼠身上定植，这表明该项目的最初计划不会成功。因此，尝试了另一种方案，其中B。通过引入B的基因产生定殖于大鼠的百日咳衍生物。支气管败血症，显示出广泛的宿主范围。我认为赋予B广泛宿主范围的基因。支气管败血症和确定宿主特异性可以通过这种策略来鉴定。