Identification of the receptor for Bordetella dermonecrotic toxin.

博德特氏菌皮肤坏死毒素受体的鉴定。

• 批准号: 21790417
• 负责人: FUKUI Aya
• 金额: $ 2.75万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790417/
• 关键词: ボルデテラ属細菌; 細菌毒素; 受容体

The aim of this project was to identify a receptor for Bordetella dermonecrotic toxin (DNT). I have determined the minimum region of DNT for cell binding, and attempted to isolate the receptor by using the synthetic peptide corresponding to the region. The functional receptor remains unknown and further trials would be needed to identify it. While attempting to purify the receptor, I found that DNT associates with the extracellular matrix. This association may facilitate the toxin action on target cells in vivo.

该项目的目的是鉴定博德特氏菌皮肤坏死毒素 (DNT) 的受体。我确定了DNT与细胞结合的最小区域，并尝试使用与该区域相对应的合成肽来分离受体。功能受体仍然未知，需要进一步的试验来识别它。在尝试纯化受体时，我发现 DNT 与细胞外基质结合。这种关联可能促进体内毒素对靶细胞的作用。

项目成果

Bordetella dermonecrotic toxin binds to target cells via the N-terminal 30 amino acids

• DOI: 10.1111/j.1348-0421.2010.00300.x
• 发表时间: 2011-03-01
• 期刊: MICROBIOLOGY AND IMMUNOLOGY
• 影响因子: 2.6
• 作者: Fukui-Miyazaki, Aya;Ohnishi, Shinya;Horiguchi, Yasuhiko
• 通讯作者: Horiguchi, Yasuhiko

Clostridium perfringens Enterotoxin Interacts with Claudins via Electrostatic Attraction

• DOI: 10.1074/jbc.m109.051417
• 发表时间: 2010-01-01
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Kimura, Jun;Abe, Hiroyuki;Horiguchi, Yasuhiko
• 通讯作者: Horiguchi, Yasuhiko