Preparation of well-controlled 2D assemblies of DNA origami using Langmuir-Blodgett technique

该项目的最初目的是利用液-气界面作为平台，将DNA折纸构建成各种2D组件。然而，在这项研究中，我们发现了一种意想不到但有趣的纳米片组装行为，这成为我们新的研究重点。2012年，我们开发了一种新的策略，通过与带相反电荷的聚电解质的界面络合来指导纳米片的组装。利用这种方法，我们演示了纳米片组装成各种宏观结构，如薄膜、纤维和胶囊。2013年，我们进一步发现，聚电解质的扩散可以驱动纳米片络合物连续生长成三维多孔泡沫状框架，其孔隙度易于调节。基于这一发现，我们提出了一种新的组装技术，即“扩散驱动逐层组装”，并展示了如何使用它来创建具有良好形状和性能控制的各种纳米片结构。

The original aim of this project was to utilize liquid-air interfaces as a platform to construct DNA origami into diverse 2D assemblies. However, during this study we discovered an unexpected but interesting assembly behavior of nanosheets, which became our new focus. In 2012, we developed a novel strategy for directing the assembly of nanosheets through interfacial complexation with oppositely charged polyelectrolytes. Using this method, we demonstrated the assembly of nanosheets into various macrostructures such as films, fibers, and capsules.In 2013, we further found that the diffusion of polyelectrolyte could drive continuous growth of the nanosheet complex into 3D porous foam-like frameworks which porosity could be easily adjusted. From such discovery, we proposed a new assembly technique, namely "diffusion driven layer-by-layer assembly", and showed how it can be used for creating diverse nanosheet architectures with good control in shape and properties.