Preparation of well-controlled 2D assemblies of DNA origami using Langmuir-Blodgett technique

该项目的最初目标是利用液-气界面作为平台，将DNA折纸构建成不同的2D组件。然而，在这项研究中，我们发现了一种意想不到但有趣的纳米片材的组装行为，这成为我们新的关注焦点。2012年，我们开发了一种新的策略，通过与相反电荷的聚电解质的界面络合来指导纳米片材的组装。利用这种方法，我们展示了纳米片材组装成各种宏观结构，如薄膜、纤维和胶囊。2013年，我们进一步发现，聚电解质的扩散可以推动纳米片材复合体的连续生长，形成孔隙率可以很容易调节的三维多孔泡沫状骨架。根据这一发现，我们提出了一种新的组装技术，即扩散驱动的逐层组装，并展示了如何利用它来创建形状和性能都得到良好控制的多样化的纳米片状结构。

The original aim of this project was to utilize liquid-air interfaces as a platform to construct DNA origami into diverse 2D assemblies. However, during this study we discovered an unexpected but interesting assembly behavior of nanosheets, which became our new focus. In 2012, we developed a novel strategy for directing the assembly of nanosheets through interfacial complexation with oppositely charged polyelectrolytes. Using this method, we demonstrated the assembly of nanosheets into various macrostructures such as films, fibers, and capsules.In 2013, we further found that the diffusion of polyelectrolyte could drive continuous growth of the nanosheet complex into 3D porous foam-like frameworks which porosity could be easily adjusted. From such discovery, we proposed a new assembly technique, namely "diffusion driven layer-by-layer assembly", and showed how it can be used for creating diverse nanosheet architectures with good control in shape and properties.