Preparation of well-controlled 2D assemblies of DNA origami using Langmuir-Blodgett technique

该项目的最初目的是利用液-气界面作为平台，将DNA折纸构建成不同的2D组件。然而，在这项研究中，我们发现了一个意想不到的，但有趣的组装行为的纳米片，这成为我们的新焦点。2012年，我们开发了一种通过与带相反电荷的聚电解质的界面复合来引导纳米片组装的新策略。使用这种方法，我们展示了纳米片组装成各种宏观结构，如薄膜，纤维和胶囊。在2013年，我们进一步发现，纳米片复合物的扩散可以驱动连续生长成3D多孔泡沫状框架，其孔隙率可以很容易地调节。从这样的发现，我们提出了一种新的组装技术，即“扩散驱动的层层组装”，并展示了它如何可以用于创建不同的纳米片结构，具有良好的控制形状和性能。

The original aim of this project was to utilize liquid-air interfaces as a platform to construct DNA origami into diverse 2D assemblies. However, during this study we discovered an unexpected but interesting assembly behavior of nanosheets, which became our new focus. In 2012, we developed a novel strategy for directing the assembly of nanosheets through interfacial complexation with oppositely charged polyelectrolytes. Using this method, we demonstrated the assembly of nanosheets into various macrostructures such as films, fibers, and capsules.In 2013, we further found that the diffusion of polyelectrolyte could drive continuous growth of the nanosheet complex into 3D porous foam-like frameworks which porosity could be easily adjusted. From such discovery, we proposed a new assembly technique, namely "diffusion driven layer-by-layer assembly", and showed how it can be used for creating diverse nanosheet architectures with good control in shape and properties.