In vivo dendritic calcium imaging of a single neuron

我们利用一种新型的多光子激光显微镜建立了树突钙成像方法。利用多种技术对以下几点的研究取得了进展。我们证实了小鼠初级体感皮层（S1）和次级运动皮层（M2）之间的相互投射。我们发现来自M2的输入引起S1中选择性第5层神经元的放电，这是在树突突之后。在与小鼠触觉感知相关的行为实验中，我们发现光遗传学方法抑制M2输入干扰了触觉感知。从这项研究中，我们发现树突钙反应是感知的基本组成部分的组成部分。

We established calcium imaging methods from dendrites using a new multi-photon laser microscope. There is the progress of research on the following points using multiple techniques. We confirmed that the reciprocal projection between the primary somatosensory cortex (S1) and the secondary motor cortex (M2) in mouse. We revealed that the input from M2 causes firing of selective layer 5 neurons in the S1, which is following dendritic spikes. From behavioral experiments, which is related with haptic perception in mouse, we found that an inhibition of M2 inputs with the optogenetical methods disturbed the haptic perception. From this study, we have found that the dendritic calcium response is a building block of a basic component of the perception.