The development of novel hepatic artery injection therapy for the hepatocellular carcinoma using metal nanoparticle with irradiation of external energy in animal model

由于肝细胞癌通常是在病毒感染或酒精性肝炎等障碍肝的基础上发展起来的，因此有必要考虑转移、复发和再治疗。因此，其治疗应具有高治愈率和微创性。本研究的目的是利用磁铁矿或二氧化钛等金属纳米颗粒进行辐射（磁场或超声波）治疗肝细胞癌。采用肿瘤移植模型和DEN癌变模型。动态CT证实DEN癌变模型中肿瘤以动脉为主。建立了大鼠肝动脉注射的手术方法。证实了磁性纳米粒子磁场产热和二氧化钛超声波自由基产热。在皮下肿瘤模型中观察到二氧化钛对肿瘤生长的抑制作用。

Because the hepatocellular carcinoma usually is developed based on the obstacle livers such as virus infection or the alcohol hepatitis, it is necessary to consider a metastasis, a recurrence and the re-treatment. Therefor, its treatment should have high curability and be less invasive. The purpose of this study is to develop the new therapy for the hepatocellular carcinoma using metal nanoparticles such as magnetite or titanium dioxide with irradiation (magnetic field or supersonic wave). The cancer transplant model and the DEN carcinogenesis model were used. The artery predominance of the tumor in the DEN carcinogenesis model was confirmed by dynamic CT. The surgical procedure of hepatic artery injection in rat was established. The generation of heat by magnetic field with magnetic nanoparticles and free radical by a supersonic wave with titanium dioxide were confirmed. The suppression of tumor growth was observed in the subcutaneous tumor model with titanium dioxide.