Sense Codon Reassignment

正义密码子重新分配

• 批准号: 24710232
• 负责人: MUKAI Takahito
• 金额: $ 3万
• 依托单位: Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24710232/
• 关键词: バイオテクノロジー

The flexibility of the standard genetic code in a modern organism Escherichia coli was experimentally evaluated. In this study, methods for the extensive engineering of the genome and codon-decoding factors were newly established. E. coli cells with only few dozens of genetic modifications accepted the reassignment of AGG to homoarginine. Since six essential genes containing eleven AGG codons were functionally expressed, it was indicated that homoarginine and arginine were largely compatible. I have previously demonstrated that E. coli cells with ten mutations accepted the reassignment of a stop codon TAG. Thus, the genetic code is still flexible, probably because the proteome is intrinsically more tolerant to the altered assignment than believed.

标准遗传密码的灵活性在现代有机体大肠杆菌实验评估。在这项研究中，基因组的广泛工程和密码子解码因子的新方法建立。只有几十个基因修饰的大肠杆菌细胞接受了AGG对同型精氨酸的重新分配。由于含有11个AGG密码子的6个必需基因功能表达，表明同型精氨酸和精氨酸在很大程度上是相容的。我之前已经证明了带有十个突变的大肠杆菌细胞接受了终止密码子TAG的重新分配。因此，遗传密码仍然是灵活的，可能是因为蛋白质组本质上比人们认为的更能容忍改变的分配。

项目成果

理研SSBC 拡張遺伝暗号システム研究チーム

RIKEN SSBC扩展遗传密码系统研究团队

Materials for transfer

转让材料

細胞の遺伝暗号改変と、これからの課題

细胞遗传密码的修改和未来的挑战

• 发表时间: 2012
• 作者: 向井崇人;大竹和正;坂本健作
• 通讯作者: 坂本健作