Multifunctional molecular structures for biotechnology and clinical diagnostics

申请人以前的研究表明，可以使用可逆化学反应作为检测分析相关生物分子的手段。在该项目中，将开发新的吸光度和荧光染料，可以与分析物的特定官能团（例如糖或抗生素的羟基）进行可逆化学反应，从而改变颜色。此外，这些染料显示基团，允许与选定的分析物分子周围的未着色单体一起聚合。染料能够与分析物发生化学相互作用的事实允许在聚合过程中获得三维自组装。在将分析物从印迹聚合物中释放出来后，产生的传感器材料选择性地识别分析物，并且与染料的可逆化学反应导致吸光度或发光强度的显着变化。通过使用不同的分析物对聚合物进行压印，可以得到对特定醇类、糖类、芳香物质或药物具有高选择性的材料。传感器层和纳米颗粒将在选择性、灵敏度、响应、操作和保质期方面进行表征，随后将对生物技术和医学研究中的分析监测进行评估。对新型传感器材料的研究将为自组织结构的发展提供见解，并允许在纳米尺度上研究化学反应。

The applicant's previous research has shown that it is possible to use reversible chemical reactions as a means to detect analytically relevant biomolecules. In the project, new absorbance and fluorescence based dyes will be developed that can perform reversible chemical reactions with specific functional groups of the analyte (e.g. hydroxyl groups of saccharides or antibiotics) causing the colour to change. Additionally, these dyes exhibit groups which allow polymerisation together with uncoloured monomers around selected analyte molecules. The fact that the dyes are capable of chemically interacting with the analyte allows to obtain three-dimensional self-assemblies during the polymerisation process. After releasing the analyte from the imprinted polymer, the resulting sensor material selectively recognises the analyte, and the reversible chemical reaction with the dye causes significant changes in absorbance or luminescence intensity. By using different analytes to imprint the polymer, materials with high selectivity for specific alcohols, saccharides, aroma substances or drugs will be obtained. The sensor layers and nanoparticles will be characterised in terms of selectivity, sensitivity, response, operational and shelf life and, subsequently will be evaluated for analytemonitoring in biotechnology and medical research. The research on the new sensor materials will give insight in the development of self-organising structures and allows to investigate chemical reactions in the nanometer scale.