酵母ミトコンドリアタンパク質の外膜挿入機構の解析

酵母线粒体蛋白外膜插入机制分析

• 批准号: 15J05033
• 负责人: 宋 紀瑶
• 金额: $ 1.09万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2015
• 资助国家: 日本
• 起止时间: 2015-04-24 至 2017-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15J05033/
• 关键词: Tom40; mitochondria; yeast; Tom40 channel

Whether the Tom40 channel, which comprise of β-barrel, can be opened laterally has been a hot topic for the past decades. We provided proofs for this possibility. In vitro, we observed fusion proteins with cytochrome b2 holding Om45 pathway targeting signal from the IMS, followed by DHFR for preventing the fusion proteins to go through the Om45 pathway, were inserted into the OM and left the Tom40 channel gradually. In vivo, we observed growth recover from yeast expressing fusion proteins with matrix targeted non-stop protein holding the targeting signal of Om45 pathway, which suggests that non-stop fusion protein does not occupy the Tom40 channel. Proving the possibility of the lateral gating of the Tom40 channel has great impact on understanding the import of mitochondrial proteins.

Tom 40通道是由β-桶组成的，它是否可以横向打开一直是人们研究的热点。我们为这种可能性提供了证据。在体外，我们观察到带有细胞色素b2的融合蛋白携带来自IMS的Om 45通路靶向信号，然后用DHFR阻止融合蛋白通过Om 45通路，插入OM并逐渐离开Tom 40通道。在体内，我们观察到从酵母表达融合蛋白的生长恢复，其中基质靶向的非终止蛋白保持Om 45通路的靶向信号，这表明非终止融合蛋白不占据Tom 40通道。证明Tom 40通道的侧向门控可能性对于理解线粒体蛋白质的输入具有重要意义。

项目成果

Analysis of the outer membrane insertion mechanism of yeast mitochondrial proteins

酵母线粒体蛋白外膜插入机制分析

• 发表时间: 2016
• 作者: Jiyao Song;Tohru Yoshihisa;Yasushi Tamura & Toshiya Endo
• 通讯作者: Yasushi Tamura & Toshiya Endo