Model Systems Core

模型系统的核心延续了匹兹堡肾脏研究中心在使用创新技术方面的丰富历史 推动肾脏生理学和病理生理学的最新概念进展的方法。这样做的目的是 生物医学资源的核心是提供多样化的创新模型系统，将允许肾脏 研究人员探索正常和疾病状态下肾脏的发育、功能和系统生理学。这个 核心分为三个亚核，从简单的单细胞生物体延伸到复杂的整个动物 模特们。利用遗传易驯化的酿酒酵母生物，酵母模型亚核 提供了一个分析保守基因的工具箱，这些基因在肾脏生理学和 蛋白质平衡。上皮模型亚核提供了高保真永生化的近端和远端 肾小管细胞系、原代细胞分离和最新的人类肾脏器官类化合物 疾病模型、肾单位发育、新陈代谢、功能和损伤反应的分析。《野兽》 Models Subcore提供了广泛的方法来评估啮齿动物肾脏的生理和 病理生理学、相关心血管变量、线粒体健康和生物能量学以及嘌呤 代谢组学。使用由其现有调查人员支持的基础设施和生产力记录， 模型系统核心将与生理学核心和KIDNIT资源开发无缝合作 核心，以便于发现。作为联合体的一部分，核心将确保方便地交换工具， 技术和专业中心资源，以促进全国范围内的肾脏研究。因此，核心 欢迎所有合作者，提供推广Subcore工具和试剂的机制，提供实践经验 指导(即迷你休假)，并将促进与个别调查人员的协作资源共享 以及与整个财团的合作。适用于对肾脏不熟悉的早期研究人员和已建立的PI 优先考虑研究、关键资源、培训和协作机会，以培养积极性和 进入一个活跃的研究渠道。核心还将提供专家意见和援助，以获得 初步数据，以支持新的和创新的赠款申请。严格的质量控制措施将确保 分核心产出的严格性和可重复性，以及正式的评价和反馈过程允许 定期重新评估核心资源的有用性。通过提供有影响力的最先进的和容易的 跨不同型号系统的可访问性服务，核心将为 肾脏研究社区，将推动基于团队的调查和催化发现。

The Model Systems Core continues the Pittsburgh Center for Kidney Research’s rich history of using creative approaches to drive the latest conceptual advances in kidney physiology and pathophysiology. The goal of this biomedical resource Core is to provide a diverse array of innovative model systems that will allow kidney researchers to explore renal development, function, and systemic physiology in normal and disease states. The Core is divided into three Subcores that extend from simple unicellular organisms to complex whole animal models. Taking advantage of the genetically tractable organism S. cerevisiae, the Yeast Models Subcore provides a toolbox for the analysis of conserved genes that play essential roles in kidney physiology and proteostasis. The Epithelial Models Subcore provides access to high fidelity immortalized proximal and distal tubule cell lines, primary cell isolations, and state-of-the art human kidney organoids for the development of disease models, analysis of nephron development, metabolism, function, and response to injury. The Animal Models Subcore provides a broad repertoire of methods to evaluate rodent kidney physiology and pathophysiology, associated cardiovascular variables, mitochondrial health and bioenergetics, and purine metabolomics. Using an infrastructure supported by its established investigators and record of productivity, the Model Systems Core will work seamlessly with the Physiology Core and the KIDNIT Resource Development Core to facilitate discovery. As part of the Consortium, the Core will ensure a facile exchange of tools, technologies, and specialized Center resources to advance kidney research nationwide. Accordingly, the Core welcomes all collaborators, offers mechanisms to promote Subcore tools and reagents, provides hands-on instruction (i.e., mini-sabbaticals), and will facilitate collaborative resource sharing with individual investigators as well as with the Consortium at large. For early-stage investigators and established PIs who are new to kidney research, key resources, training, and collaborative opportunities will be prioritized to cultivate enthusiasm and entry into an active research pipeline. The Core will also provide expert advice and aid in the acquisition of preliminary data to support new and innovative grant applications. Stringent quality control measures will ensure the rigor and reproducibility of Subcore outputs, and a formalized evaluation and feedback process permits regular reassessment of the usefulness of Core resources. By offering impactful state-of-the-art and readily accessible services across diverse model systems, the Core will provide a unique national resource for the kidney research community that will propel team-based investigation and catalyze discovery.