Model Systems Core

模型系统核心

• 批准号: 10747626
• 负责人: AROHAN R SUBRAMANYA
• 金额: $ 29.59万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10747626
• 关键词: Acceleration; Acids; Animal Model; Animals; Applications Grants; Bioenergetics; Biological Models; Blinded; CRISPR/Cas technology; Cardiovascular system; Catalogs; Cell Culture Techniques; Cell Line; Cell Separation; Cell Volumes; Cells; Collaborations; Communities; Complex; Computer Models; Creativeness; Data; Data Set; Development; Disease; Disease model; Distal; Ensure; Environment; Epithelium; Evaluation; Experimental Designs; Feedback; Functional disorder; Generations; Genetic study; Genotype; Goals; Grant; Health; Homeostasis; Hormone Responsive; Human; Individual; Infrastructure; Instruction; Investigation; Journals; Kidney; Kidney Diseases; Leadership; Liquid substance; Measures; Metabolism; Methods; Mitochondria; Modeling; Monitor; Mus; Nephrons; Organism; Organoids; Output; Peer Review; Pharmaceutical Preparations; Physiology; Play; Process; Productivity; Protocols documentation; Publications; Purines; Quality Control; Reagent; Recording of previous events; Renal function; Reproducibility; Research; Research Personnel; Resolution; Resource Development; Resource Sharing; Resources; Rodent; Rodent Model; Role; Saccharomyces cerevisiae; Scientist; Services; Specialized Center; System; Technology; Testing; Training; Validation; Work; Yeast Model System; Yeasts; absorption; base; biomedical resource; cell immortalization; cell type; design; experimental study; gene conservation; hands on instruction; induced pluripotent stem cell; innovation; kidney cell; meetings; member; metabolomics; nephrogenesis; new technology; next generation; proteostasis; recruit; response to injury; sabbatical; skills; social media; solute; tool; web site

The Model Systems Core continues the Pittsburgh Center for Kidney Research’s rich history of using creative approaches to drive the latest conceptual advances in kidney physiology and pathophysiology. The goal of this biomedical resource Core is to provide a diverse array of innovative model systems that will allow kidney researchers to explore renal development, function, and systemic physiology in normal and disease states. The Core is divided into three Subcores that extend from simple unicellular organisms to complex whole animal models. Taking advantage of the genetically tractable organism S. cerevisiae, the Yeast Models Subcore provides a toolbox for the analysis of conserved genes that play essential roles in kidney physiology and proteostasis. The Epithelial Models Subcore provides access to high fidelity immortalized proximal and distal tubule cell lines, primary cell isolations, and state-of-the art human kidney organoids for the development of disease models, analysis of nephron development, metabolism, function, and response to injury. The Animal Models Subcore provides a broad repertoire of methods to evaluate rodent kidney physiology and pathophysiology, associated cardiovascular variables, mitochondrial health and bioenergetics, and purine metabolomics. Using an infrastructure supported by its established investigators and record of productivity, the Model Systems Core will work seamlessly with the Physiology Core and the KIDNIT Resource Development Core to facilitate discovery. As part of the Consortium, the Core will ensure a facile exchange of tools, technologies, and specialized Center resources to advance kidney research nationwide. Accordingly, the Core welcomes all collaborators, offers mechanisms to promote Subcore tools and reagents, provides hands-on instruction (i.e., mini-sabbaticals), and will facilitate collaborative resource sharing with individual investigators as well as with the Consortium at large. For early-stage investigators and established PIs who are new to kidney research, key resources, training, and collaborative opportunities will be prioritized to cultivate enthusiasm and entry into an active research pipeline. The Core will also provide expert advice and aid in the acquisition of preliminary data to support new and innovative grant applications. Stringent quality control measures will ensure the rigor and reproducibility of Subcore outputs, and a formalized evaluation and feedback process permits regular reassessment of the usefulness of Core resources. By offering impactful state-of-the-art and readily accessible services across diverse model systems, the Core will provide a unique national resource for the kidney research community that will propel team-based investigation and catalyze discovery.

模型系统核心延续了匹兹堡肾脏研究中心使用创意的丰富历史 推动肾脏生理学和病理生理学最新概念进展的方法。此举的目标 生物医学资源的核心是提供多样化的创新模型系统，使肾脏 研究人员探索正常和疾病状态下的肾脏发育、功能和全身生理学。这 核心分为三个子核心，从简单的单细胞生物延伸到复杂的整体动物 模型。利用遗传上易驯化的有机体酿酒酵母，酵母模型子核心 提供了一个用于分析在肾脏生理学中发挥重要作用的保守基因的工具箱 蛋白质稳态。上皮模型子核心提供高保真永生化近端和远端 肾小管细胞系、原代细胞分离和最先进的人类肾脏类器官，用于开发 疾病模型，肾单位发育、代谢、功能和损伤反应的分析。动物 Models Subcore 提供了广泛的方法来评估啮齿动物肾脏生理学和 病理生理学、相关心血管变量、线粒体健康和生物能量学以及嘌呤 代谢组学。利用由其既定调查员和生产力记录支持的基础设施， 模型系统核心将与生理学核心和 KIDNIT 资源开发无缝协作 促进发现的核心。作为联盟的一部分，核心将确保工具的便捷交换， 技术和专业中心资源，以推进全国肾脏研究。因此，核心 欢迎所有合作者，提供推广 Subcore 工具和试剂的机制，提供实践机会 指导（即小型休假），并将促进与个别研究人员的协作资源共享 以及整个财团。对于刚接触肾脏的早期研究人员和已建立的 PI 将优先考虑研究、关键资源、培训和合作机会，以培养热情和 进入活跃的研究管道。核心还将在收购方面提供专家建议和帮助 支持新的和创新的赠款申请的初步数据。严格的质量控制措施将确保 子核心输出的严谨性和可重复性，以及正式的评估和反馈流程允许 定期重新评估核心资源的有用性。通过提供有影响力的、最先进的、容易的 跨不同模型系统的无障碍服务，核心将为 肾脏研究社区将推动基于团队的调查并促进发现。