Mechanisms of Target of Rapamycin Complex 1 Dependent Epigenetic Regulation

雷帕霉素复合物1依赖的表观遗传调控靶点机制

• 批准号: 10515603
• 负责人: Ronald Laribee
• 金额: $ 30.8万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10515603
• 关键词: Acetylation; Address; Affect; Architecture; Binding; Biochemical; Cell Death; Cell Nucleus; Cell Proliferation; Cell Survival; Cells; ChIP-seq; Chromatin; Chromatin Structure; Complex; Coupled; Critical Pathways; Cytoplasm; Deacetylase; Disease; Engineering; Environment; Epigenetic Process; FRAP1 gene; Future; Gene Expression; Genes; Genetic; Genetic Screening; Genetic Transcription; Genomic approach; Goals; HMGB Proteins; HMGB1 gene; Health; Histone Acetylation; Histone Deacetylase; Histone H3; Histone H4; Histones; Homeostasis; Homologous Gene; Human; Immune signaling; In Vitro; Individual; Inflammation; Knowledge; Libraries; Link; Lysine; Malignant Neoplasms; Metabolic; Metabolic Control; Metabolism; Mitochondria; Modeling; Nutrient; Obesity; Outcome; Pathologic; Pathway interactions; Peptides; Phenotype; Post-Translational Protein Processing; Process; Proteins; Proteomics; Reader; Regulation; Role; Signal Pathway; Signal Transduction; Sirolimus; Sirtuins; Site; Stress; Testing; Transcriptional Regulation; Work; Yeast Model System; Yeasts; analog; cell growth; chemical genetics; epigenetic regulation; epigenome; genetic analysis; histone modification; inhibitor; mutant; novel; prevent; success; tumorigenesis

Project Summary Environmental nutrient availability and metabolism profoundly affects an individual’s health, while deregulation of nutrient signaling contributes to many diseases, including cancer. Nutrient signaling and metabolism regulate the epigenome to affect cellular phenotype and function, yet mechanisms explaining how nutrients signal to the epigenome are lacking. Defining these mechanisms constitutes a critical scientific problem that is essential to address. By defining these mechanisms, we will understand how nutrient exposures affect health, and how aberrant nutrient signaling causes disease. The mechanistic target of rapamycin complex 1 (mTORC1) is an evolutionarily conserved nutrient activated signaling pathway. MTORC1 responds to diverse nutrient and metabolic inputs to promote cell growth and proliferation, and it is deregulated in cancer and other diseases. While mTORC1 is an emerging epigenetic regulator, how it signals to the epigenome is unknown. In this project, we will use a yeast model to build on our previous successes to define these mechanisms. Herein, we will test the overarching hypothesis that TORC1 signaling controls the chromatin binding of architectural proteins and histone reader proteins that maintain viability during nutrient stress and regulate metabolic gene expression. In Aim I, we will identify specific epigenetic pathways acting on histone H3 that promote binding of high mobility group box (HMGB) proteins to chromatin to prevent cell death under nutrient stress conditions. We then will define biochemically and genetically how non-chromatin bound HMGB proteins cause cell death during TORC1 stress. Stressed human cells evict HMGB1 from chromatin to affect cytoplasmic metabolic activities, initiate innate immune signaling and inflammation, and promote tumorigenesis. These yeast studies will identify conserved epigenetic pathways that are critical for retaining HMGB1 on chromatin during mTORC1 stress to prevent such HMGB1-induced pathological effects. Aim II will use proteomic and genomic approaches to define how yeast TORC1 represses conserved sirtuin histone deacetylase activity to regulate histone reader chromatin binding and control mitochondrial metabolic transcription. We then will perform mechanistic studies to assess how these histone reader proteins transcriptionally regulate metabolic gene expression. By the project’s conclusion, we will have defined novel and conserved mechanisms used by TORC1 to modify the epigenome, which prevent cell death during nutrient stress and regulate metabolic gene transcription. These mechanisms will be directly relevant for understanding how human mTORC1 deregulation alters the epigenome to cause disease.

项目摘要 环境养分的可获得性和新陈代谢深刻地影响着个人的健康，而放松管制 营养信号的传递导致了许多疾病，包括癌症。营养信号与新陈代谢 调节表观基因组以影响细胞表型和功能，但解释营养物质 缺乏对表观基因组的信号。定义这些机制构成了一个关键的科学问题，即 必须解决的问题。通过定义这些机制，我们将了解营养暴露如何影响健康， 以及营养信号异常是如何导致疾病的。雷帕霉素复合体1的作用靶点 (MTORC1)是一条进化保守的营养激活信号通路。MTORC1对多样化的回应 营养和代谢输入促进细胞生长和增殖，它在癌症和其他疾病中被解除调控 疾病。虽然mTORC1是一种新兴的表观遗传调控因子，但它如何向表观基因组发出信号尚不清楚。在……里面 在这个项目中，我们将使用酵母模型来构建我们以前的成功来定义这些机制。在这里， 我们将测试TORC1信号控制体系结构染色质结合的总体假设 在营养胁迫中维持活性并调节代谢基因的蛋白质和组蛋白阅读器蛋白 表情。在目标I中，我们将确定作用于组蛋白H3的特定表观遗传通路，这些通路促进组蛋白H3的结合 高迁移率族框(HMGB)蛋白到染色质，以防止细胞在营养胁迫条件下死亡。 然后，我们将从生化和遗传学的角度定义非染色质结合的HMGB蛋白如何导致细胞死亡 在TORC1应激期间。应激状态下人类细胞从染色质中排出HMGB1影响细胞质代谢 活动，启动先天免疫信号和炎症，并促进肿瘤的形成。这些酵母菌研究 将确定在mTORC1期间将HMGB1保留在染色质上的关键的保守的表观遗传途径 应激以防止这种HMGB1诱导的病理效应。AIM II将使用蛋白质组和基因组 确定酵母TORC1如何抑制保守的sirtuin组蛋白脱乙酰酶活性来调节的方法 组蛋白阅读器与染色质结合，控制线粒体代谢转录。然后我们将表演 评估这些组蛋白阅读器蛋白如何转录调节代谢基因的机制研究 表情。通过项目的结论，我们将定义新的和保守的机制使用 TORC1修饰表观基因组，防止细胞在营养胁迫下死亡并调节代谢基因 抄写。这些机制将与理解人类mTORC1如何放松管制直接相关 改变表观基因组以致病。