Analysis of new muscle specific genes/proteins and their function in sarcomere assembly in C. elegans using SAGE and Affymetrix Microarray Chip technology including Bioinformatics

使用 SAGE 和 Affymetrix 微阵列芯片技术（包括生物信息学）分析新的肌肉特异性基因/蛋白质及其在秀丽隐杆线虫肌节组装中的功能

• 批准号: 5442584
• 负责人: Barbara Meissner
• 金额: --
• 依托单位: Department of Zoology
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2005-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5442584?language=en
• 关键词: Analysis; new; muscle; specific; genes

The aim of my research proposal is to identify novel genes/proteins involved in sarcomere assembly in C. elegans. With the fellowship I am applying for, I will work on a project in the laboratory of Prof. Don Moerman at the University of British Columbia, Vancouver. His lab focuses on muscle development and specifically on the problem of muscle sarcomere assembly in C. elegans. This system is an excellent in vivo model to study the assembly of integrin-containing adhesion structures. Recently, a large number of muscle specific genes have been identified by serial analysis of gene expression (SAGE) using C. elegans muscle specific RNA at the Michael Smith Genome Sciences Center, Vancouver. I will use this database to help find new genes/proteins placed in adhesion complexes and to determine their function during sarcomere assembly. The specific goals of the proposed research are as follows: 1) Determination of the expression pattern of different candidate genes in C. elegans focusing on subcellular localization. 2) Generating C. elegans mutant animals for the genes of interest using RNAi or a mutagenesis screen. 3) Phenotyp analysis of mutant animals focusing on sarcomere development. 4) Biochemical analysis of interesting target proteins. Since there are many genes having human homologues with unknown function the findings will be very useful as myofilament lattice assembly in the nematode is remarkably similar to the assembly of focal adhesions in mammalian cell culture.

我的研究计划的目的是确定新的基因/蛋白质参与肌节组装在C。优雅的有了我申请的奖学金，我将在温哥华的不列颠哥伦比亚省的Don Moerman教授的实验室里从事一个项目。他的实验室专注于肌肉发育，特别是C。优美的该系统是研究含整合素的粘附结构组装的极好的体内模型。近年来，利用C. elegans肌肉特异性RNA，位于温哥华的Michael Smith基因组科学中心。我将使用这个数据库来帮助寻找位于粘附复合物中的新基因/蛋白质，并确定它们在肌节组装过程中的功能。具体研究目标如下：1）确定不同候选基因在C. elegans专注于亚细胞定位。2)生成C.使用RNA干扰或突变筛选对秀丽隐杆线虫突变动物进行感兴趣的基因。3)肌节发育突变动物的表型分析。4)感兴趣的靶蛋白的生化分析。由于有许多基因具有人类同源物与未知功能的研究结果将是非常有用的，因为线虫中的肌丝网格组装与哺乳动物细胞培养物中的粘着斑组装非常相似。